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利用新克隆的α卫星DNA探针进行的人类3号染色体着丝粒研究。

Studies on the human chromosome 3 centromere with a newly cloned alphoid DNA probe.

作者信息

Delattre O, Bernard A, Malfoy B, Marlhens F, Viegas-Pequignot E, Brossard C, Haguenauer O, Creau-Goldberg N, N'guyen V C, Dutrillaux B

机构信息

Section de Biologie, Institut Curie, Paris, France.

出版信息

Hum Hered. 1988;38(3):156-67. doi: 10.1159/000153777.

DOI:10.1159/000153777
PMID:2899543
Abstract

Starting from a chromosome-specific DNA library, we have isolated a human chromosome-specific satellite DNA sequence. This sequence of 635 base pairs (bp) consists of 3.7 alpha DNA monomers of 170-171 bp. Under high stringency it hybridizes to the centromere of chromosome 3 in a region composed of 2,750 bp tandem repeats characterized by the regular spacing of Hind III and TaqI restriction enzyme recognition sites. It has diverged and undergone amplification after the human speciation. The amplification allows an easy monitoring of the chromosome 3 centromere by in situ hybridization with a nonradioactive probe.

摘要

从一个染色体特异性DNA文库开始，我们分离出了一个人类染色体特异性卫星DNA序列。这个由635个碱基对（bp）组成的序列包含3.7个170 - 171 bp的αDNA单体。在高严格度条件下，它与3号染色体的着丝粒杂交，该区域由2750 bp的串联重复序列组成，其特征是Hind III和TaqI限制酶识别位点有规律的间隔。它在人类物种形成后发生了分化并经历了扩增。这种扩增使得通过用非放射性探针进行原位杂交能够轻松监测3号染色体着丝粒。

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1
Studies on the human chromosome 3 centromere with a newly cloned alphoid DNA probe.利用新克隆的α卫星DNA探针进行的人类3号染色体着丝粒研究。
Hum Hered. 1988;38(3):156-67. doi: 10.1159/000153777.
2
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Chromosome-specific alpha satellite DNA from human chromosome 1: hierarchical structure and genomic organization of a polymorphic domain spanning several hundred kilobase pairs of centromeric DNA.来自人类1号染色体的染色体特异性α卫星DNA：跨越数百千碱基对着丝粒DNA的多态性结构域的层次结构和基因组组织。
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Detection of restriction fragment length polymorphisms at the centromeres of human chromosomes by using chromosome-specific alpha satellite DNA probes: implications for development of centromere-based genetic linkage maps.利用染色体特异性α卫星DNA探针检测人类染色体着丝粒处的限制性片段长度多态性：对着丝粒基因连锁图谱构建的意义
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引用本文的文献

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Consensus higher order repeats and frequency of string distributions in human genome.人类基因组中一致性的高级重复序列和串分布频率。
Curr Genomics. 2007 Apr;8(2):93-111. doi: 10.2174/138920207780368169.
2
Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.通过与生物素化探针进行原位杂交将单拷贝DNA序列定位到人类染色体上：利用增强荧光数字成像显微镜提高检测灵敏度。
Proc Natl Acad Sci U S A. 1989 Jan;86(2):582-6. doi: 10.1073/pnas.86.2.582.
3
Chromosome specificity of satellite DNAs: short- and long-range organization of a diverged dimeric subset of human alpha satellite from chromosome 3.
卫星DNA的染色体特异性：来自3号染色体的人类α卫星发散二聚体亚群的短程和长程组织
Chromosoma. 1989 May;97(6):475-80. doi: 10.1007/BF00295032.
4
Concerted evolution of alpha satellite DNA: evidence for species specificity and a general lack of sequence conservation among alphoid sequences of higher primates.α卫星DNA的协同进化：高等灵长类动物α卫星序列中物种特异性及普遍缺乏序列保守性的证据。
Chromosoma. 1989 Oct;98(4):273-9. doi: 10.1007/BF00327313.
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A human alpha satellite DNA subset specific for chromosome 12.一个对12号染色体特异的人类α卫星DNA亚群。
Am J Hum Genet. 1990 Apr;46(4):784-8.
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A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence.一项关于α卫星DNA在所有人类染色体上的基因组分布的调查以及一个新的共有序列的推导。
Nucleic Acids Res. 1991 Mar 25;19(6):1179-82. doi: 10.1093/nar/19.6.1179.