利用新克隆的α卫星DNA探针进行的人类3号染色体着丝粒研究。
Studies on the human chromosome 3 centromere with a newly cloned alphoid DNA probe.
作者信息
Delattre O, Bernard A, Malfoy B, Marlhens F, Viegas-Pequignot E, Brossard C, Haguenauer O, Creau-Goldberg N, N'guyen V C, Dutrillaux B
机构信息
Section de Biologie, Institut Curie, Paris, France.
出版信息
Hum Hered. 1988;38(3):156-67. doi: 10.1159/000153777.
Starting from a chromosome-specific DNA library, we have isolated a human chromosome-specific satellite DNA sequence. This sequence of 635 base pairs (bp) consists of 3.7 alpha DNA monomers of 170-171 bp. Under high stringency it hybridizes to the centromere of chromosome 3 in a region composed of 2,750 bp tandem repeats characterized by the regular spacing of Hind III and TaqI restriction enzyme recognition sites. It has diverged and undergone amplification after the human speciation. The amplification allows an easy monitoring of the chromosome 3 centromere by in situ hybridization with a nonradioactive probe.
从一个染色体特异性DNA文库开始，我们分离出了一个人类染色体特异性卫星DNA序列。这个由635个碱基对（bp）组成的序列包含3.7个170 - 171 bp的αDNA单体。在高严格度条件下，它与3号染色体的着丝粒杂交，该区域由2750 bp的串联重复序列组成，其特征是Hind III和TaqI限制酶识别位点有规律的间隔。它在人类物种形成后发生了分化并经历了扩增。这种扩增使得通过用非放射性探针进行原位杂交能够轻松监测3号染色体着丝粒。
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