Nizetić D, Stevanović M, Soldatović B, Savić I, Crkvenjakov R
Genetic Engineering Center, Belgrade, Yugoslavia.
Immunogenetics. 1988;28(2):91-8. doi: 10.1007/BF00346156.
We analyzed the restriction fragment length polymorphism of class I and class II MHC genes in DNA from 20 individuals belonging to the four different species of the complex of species of Balkan mole rats Spalax leucodon captured at four different localities in Yugoslavia. All populations were tested with four restriction enzymes and one conserved mouse probe for each of the two classes of MHC genes. The probes employed detect either limited polymorphism of class I genes or lack of polymorphic bands containing class II genes. Of the two other subterranean rodents that have been studied, four karyotype forms of the Israeli mole rat show polymorphism in both classes of MHC genes similar to the one found in all other mammals (Nizetić et al. 1985), and the Syrian hamster shows limited polymorphism of class I genes and high polymorphism of class II genes (McGuire et al. 1985). Balkan mole rats belong to a new group in this respect, different from all mammals studied so far, since they apparently show limited polymorphism of both classes of MHC genes.
我们分析了从南斯拉夫四个不同地点捕获的属于巴尔干鼹形田鼠复合体四个不同物种的20只个体的DNA中I类和II类主要组织相容性复合体（MHC）基因的限制性片段长度多态性。对所有种群使用四种限制性内切酶和针对两类MHC基因各自的一种保守小鼠探针进行检测。所使用的探针检测到I类基因的多态性有限，或者检测到缺乏含II类基因的多态性条带。在已研究的另外两种地下啮齿动物中，以色列鼹形田鼠的四种核型形式在两类MHC基因中均显示出多态性，类似于在所有其他哺乳动物中发现的多态性（尼泽蒂奇等人，1985年），而叙利亚仓鼠显示出I类基因的多态性有限和II类基因的高度多态性（麦圭尔等人，1985年）。在这方面，巴尔干鼹形田鼠属于一个新的类别，不同于迄今为止研究过的所有哺乳动物，因为它们显然在两类MHC基因中均显示出有限的多态性。
