Haveman J, Jansen W, Wondergem J, Begg A C
Department of Radiotherapy, University Hospital, Amsterdam, The Netherlands.
Int J Radiat Biol. 1988 Jul;54(1):105-13. doi: 10.1080/09553008814551541.
The skin of mouse legs was exposed to 44 degrees C hyperthermia using a thermostatically controlled waterbath. Treatment at 44 degrees C, for 15 or 30 min, led to oedema in the dermis immediately after treatment and to an infiltration by neutrophils within 7 h. The oedema disappeared in 2 days. Treatment for 60 min at 44 degrees C led to subepidermal blistering and as a result of this a considerable area of the tissue became necrotic 4 days after treatment. A repair reaction followed, and 3 weeks after heating for 60 min at 44 degrees C the epithelium was again completely or almost completely covering the underlying tissue. Shortly (7 h) after 15 or 30 min at 44 degrees C an increase was observed in the number of basal cells in the epithelium incorporating [3H]thymidine. This increase declined slowly with time: 3 weeks after treatment the number of labelled basal cells was not significantly different from that in untreated skin. Shortly after 60 min at 44 degrees C some basal cells of the epidermis still incorporated [3H]thymidine. The labelling index dropped to near-zero at day 2 after 60 min at 44 degrees C. Thereafter repopulation started and in the areas next to the granulation tissue the labelling index of basal cells reached values close to 100 per cent, 2 or 3 weeks after treatment. Treatment for 15 min at 44 degrees C did not lead to a stimulation of the proliferation of subcutaneous endothelial cells. Both 30 min and 60 min at 44 degrees C led to a greatly enhanced proportion of labelled subcutaneous endothelial cells after 2 days and 4 weeks, respectively (labelling index between 35 and 40 per cent). After this peak value the labelling index declined rapidly. However, in granulation tissue it remained high for about 10 days after the peak on day 4. The stimulated proliferation of subcutaneous endothelial cells after heating for 30 and 60 min at 44 degrees C correlated well with the finding that these heat treatments, given after or shortly before X-irradiation, led to a greatly reduced (X-ray-induced) tumour bed effect.
使用恒温控制的水浴将小鼠腿部皮肤暴露于44摄氏度的高温环境中。在44摄氏度下处理15或30分钟，处理后立即导致真皮水肿，并在7小时内出现中性粒细胞浸润。水肿在2天内消失。在44摄氏度下处理60分钟导致表皮下水疱形成，结果在处理后4天相当大的组织区域发生坏死。随后出现修复反应，在44摄氏度下加热60分钟后3周，上皮再次完全或几乎完全覆盖下方组织。在44摄氏度下处理15或30分钟后不久（7小时），观察到上皮中掺入[3H]胸腺嘧啶核苷的基底细胞数量增加。这种增加随时间缓慢下降：处理后3周，标记的基底细胞数量与未处理皮肤中的数量无显著差异。在44摄氏度下处理60分钟后不久，表皮的一些基底细胞仍掺入[3H]胸腺嘧啶核苷。在44摄氏度下处理60分钟后第2天，标记指数降至接近零。此后开始重新增殖，在肉芽组织旁边的区域，基底细胞的标记指数在处理后2或3周达到接近100%的值。在44摄氏度下处理15分钟未导致皮下内皮细胞增殖受到刺激。在44摄氏度下处理30分钟和60分钟分别在2天和4周后导致标记的皮下内皮细胞比例大大增加（标记指数在35%至40%之间）。在达到该峰值后，标记指数迅速下降。然而，在肉芽组织中，在第4天达到峰值后约10天内仍保持较高水平。在44摄氏度下加热30分钟和60分钟后皮下内皮细胞的增殖受到刺激，这与以下发现密切相关：在X射线照射之后或之前不久进行这些热处理，会导致（X射线诱导的）肿瘤床效应大大降低。
