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鸟嘌呤核苷酸对通透化AtT-20细胞促肾上腺皮质激素（ACTH）分泌的双重调节

Dual regulation of ACTH secretion by guanine nucleotides in permeabilized AtT-20 cells.

作者信息

Luini A, De Matteis M A

机构信息

Neuroendocrinology Unit, Istituto di Ricerche Farmacologiche Mario, Negri, Italy.

出版信息

Cell Mol Neurobiol. 1988 Mar;8(1):129-38. doi: 10.1007/BF00712918.

DOI:10.1007/BF00712918
PMID:2900071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11567329/
Abstract
  1. We have examined the effects of guanine nucleotides on ACTH secretion from digitonin-permeabilized AtT-20 cells, with the aim of analyzing the involvement of GTP-binding proteins (G proteins) in the secretory process. 2. AtT-20 cells permeabilized with 20 microM digitonin displayed calcium-dependent secretion. The EC50 of calcium was approximately 2 microM and the maximal stimulation was 350% of basal release. 3. Nonhydrolyzable guanine nucleotides also stimulated ACTH release, in a virtually Ca2+-free medium. The EC50 of guanosine 5'-(3-O-thio)triphosphate (GTP gamma S) was approximately 15 microM and the maximal stimulation was approximately 230% of basal release. The effects of calcium and guanine nucleotides were not additive. 4. In the presence of the inhibitory hormone, somatostatin guanine nucleotides inhibited the calcium-stimulated secretion. 5. Both the stimulatory and the inhibitory effects on secretion of guanine nucleotides were independent of changes in cyclic AMP (cAMP) and calcium. It is suggested that G proteins influence an unknown step in the secretion process, which would be near or at the exocytotic site. 6. The results can be explained by assuming the existence of two types of G proteins, one with stimulatory effects on exocytotic release (GeS) and another with inhibitory effects (GeI).
摘要
  1. 我们研究了鸟嘌呤核苷酸对经洋地黄皂苷通透处理的AtT - 20细胞促肾上腺皮质激素（ACTH）分泌的影响，目的是分析GTP结合蛋白（G蛋白）在分泌过程中的作用。2. 用20微摩尔洋地黄皂苷通透处理的AtT - 20细胞表现出钙依赖性分泌。钙的半数有效浓度（EC50）约为2微摩尔，最大刺激量为基础释放量的350%。3. 在几乎无Ca2 +的培养基中，不可水解的鸟嘌呤核苷酸也能刺激ACTH释放。鸟苷5' -（3 - O -硫代）三磷酸（GTPγS）的EC50约为15微摩尔，最大刺激量约为基础释放量的230%。钙和鸟嘌呤核苷酸的作用并非相加性的。4. 在抑制性激素生长抑素存在的情况下，鸟嘌呤核苷酸抑制钙刺激的分泌。5. 鸟嘌呤核苷酸对分泌的刺激和抑制作用均与环磷酸腺苷（cAMP）和钙的变化无关。提示G蛋白影响分泌过程中一个未知步骤，该步骤可能靠近或位于胞吐部位。6. 这些结果可以通过假设存在两种类型的G蛋白来解释，一种对胞吐释放有刺激作用（GeS），另一种有抑制作用（GeI）。

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Dual regulation of ACTH secretion by guanine nucleotides in permeabilized AtT-20 cells.鸟嘌呤核苷酸对通透化AtT-20细胞促肾上腺皮质激素（ACTH）分泌的双重调节
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THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS.螃蟹（黄道蟹）肌纤维收缩与舒张对游离钙离子胞内浓度的依赖性
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