Ghanem N, Dugoujon J M, Bensmana M, Huck S, Lefranc M P, Lefranc G
UA CNRS 1191 Génétique Moléculaire, Université des Sciences et Techniques du Languedoc, Montpellier, France.
Eur J Immunol. 1988 Jul;18(7):1067-72. doi: 10.1002/eji.1830180715.
Of the four human IgG subclass heavy chain (IGHG) genes, three--IGHG1, IGHG2 and IGHG3--have allelic forms which encode antigenic determinants called G1m, G2m and G3m allotypes for markers of the IgG1, IgG2 and IgG3 molecules. The alleles at these three closely linked loci are transmitted as Gm haplotypes, together with the IGHG4 and the IGHGP (or pseudo gamma) genes. Restriction Fragment Length Polymorphisms (RFLPs) have been described for all these IGHG genes, allowing us to define at least 15 RFLP alleles (N. Ghanem et al., Eur. J. Immunol. 1988. 18:1059). In this article, we determine unambiguously the Gm alleles and haplotypes and the associated RFLPs in French, Lebanese and Tunisian families, chosen for the various Gm phenotypes of their members, in order to correlate both these polymorphisms and to know the evolution of the IGHG multigene family. We were able to assign all the IGHG alleles to the Gm alleles and to their linked IGHG4 and IGHGP genes allowing us to define restriction fragment haplotypes of the IGHG gene family.
在四个人类IgG亚类重链（IGHG）基因中，其中三个——IGHG1、IGHG2和IGHG3——具有等位基因形式，这些等位基因编码被称为G1m、G2m和G3m同种异型的抗原决定簇，作为IgG1、IgG2和IgG3分子的标记。这三个紧密连锁位点的等位基因与IGHG4和IGHGP（或假γ）基因一起作为Gm单倍型进行传递。已经描述了所有这些IGHG基因的限制性片段长度多态性（RFLP），这使我们能够定义至少15个RFLP等位基因（N. Ghanem等人，《欧洲免疫学杂志》，1988年。18:1059）。在本文中，我们明确确定了法国、黎巴嫩和突尼斯家庭中的Gm等位基因、单倍型以及相关的RFLP，这些家庭因其成员的各种Gm表型而被选中，以便将这两种多态性相互关联，并了解IGHG多基因家族的进化。我们能够将所有IGHG等位基因与其相连的IGHG4和IGHGP基因分配给Gm等位基因，从而使我们能够定义IGHG基因家族的限制性片段单倍型。
