Maggio-Price L, Wolf N S, Priestley G V, Pietrzyk M E, Bernstein S E
Department of Pathology, University of Washington School of Medicine, Seattle 98195.
Exp Hematol. 1988 Sep;16(8):653-9.
Serial transplantation and competitive repopulation were used to evaluate any loss of self-replicative capacity of bone marrow stem cells in a mouse model with increased and persistent hemopoietic demands. Congenic marrows from old control and from young and old mice with hereditary spherocytic anemia (sphha/sphha) were serially transplanted at 35-day intervals into normal irradiated recipients. Old anemic marrow failed or reverted to recipient karyotype at a mean of 3.5 transplants, and young anemic marrow reverted at a mean of 4.0 transplants, whereas controls did so at a mean of 5.0 transplants. In a competitive assay in which a mixture of anemic and control marrow was transplanted, the anemic marrow persisted to 10 months following transplantation; anemic marrow repopulation was greater if anemic marrow sex matched with the host. It is possible that lifelong stress of severe anemia decreases stem cell reserve in the anemic sphha/sphha mouse marrow. However, marginal differences in serial transplantation number and the maintenance of anemic marrow in a competition assay would suggest that marrow stem cells, under prolonged stress, are capable of exhibiting good repopulating and self-replicating abilities.
采用连续移植和竞争性再增殖方法，在造血需求增加且持续存在的小鼠模型中，评估骨髓干细胞自我复制能力的任何丧失情况。来自老年对照小鼠以及患有遗传性球形红细胞贫血（sphha/sphha）的年轻和老年小鼠的同基因骨髓，每隔35天连续移植到正常受照受体中。老年贫血骨髓平均在3.5次移植时失败或恢复为受体核型，年轻贫血骨髓平均在4.0次移植时恢复，而对照骨髓平均在5.0次移植时恢复。在一项将贫血和对照骨髓混合移植的竞争性试验中，贫血骨髓在移植后持续存在10个月；如果贫血骨髓与宿主性别匹配，贫血骨髓的再增殖能力更强。严重贫血的终身应激可能会减少贫血sphha/sphha小鼠骨髓中的干细胞储备。然而，连续移植次数的微小差异以及竞争性试验中贫血骨髓的维持情况表明，在长期应激下，骨髓干细胞能够表现出良好的再增殖和自我复制能力。
