Naguib A M, Bansal J, Calton G J, Burnett J W
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
Toxicon. 1988;26(4):387-94. doi: 10.1016/0041-0101(88)90007-4.
A comparison of the purification of the nematocyst venom of Chironex fleckeri by affinity immunochromatography using 13 different monoclonal antibodies was made. Varying degrees of purification of mouse lethal factor, hemolysin and dermonecrotic factors, as well as antigen positive proteins were achieved with each of the monoclonal antibodies. Although the protein curves of the chromatography were similar, each of the monoclonal antibody columns had a distinctive pharmacological and SDS-PAGE profile. At least two hemolysins (120,000 and 70,000 molecular weight), two dermonecrotic principles (120,000, less than 120,000) and three lethal factors (120,000, 70,000 and 14,500 molecular weight) were detected. The degree to which aggregation and fragmentation affects the molecular weights of these proteins is not known. It appears that multiple pharmacological activities are present within the same molecule since it is only with great difficulty that a pharmacological activity can be assigned to a specific molecular weight.
对使用13种不同单克隆抗体通过亲和免疫色谱法纯化多斑曲纹唇鱼（Chironex fleckeri）刺丝囊毒液进行了比较。每种单克隆抗体对小鼠致死因子、溶血素和皮肤坏死因子以及抗原阳性蛋白都实现了不同程度的纯化。尽管色谱图的蛋白质曲线相似，但每个单克隆抗体柱都有独特的药理学和SDS-PAGE图谱。检测到至少两种溶血素（分子量分别为120,000和70,000）、两种皮肤坏死因子（120,000、小于120,000）和三种致死因子（分子量分别为120,000、70,000和14,500）。聚集和片段化对这些蛋白质分子量的影响程度尚不清楚。似乎同一分子内存在多种药理活性，因为很难将一种药理活性归属于特定的分子量。
