在海滩边分离的箱形水母（Chironex fleckeri）刺丝囊毒液的部分纯化。

Partial purification of box jellyfish (Chironex fleckeri) nematocyst venom isolated at the beachside.

作者信息

Bloom D A, Burnett J W, Alderslade P

机构信息

Department of Dermatology, University of Maryland School of Medicine, Baltimore 21201, USA.

出版信息

Toxicon. 1998 Aug;36(8):1075-85. doi: 10.1016/s0041-0101(98)00096-8.

DOI:10.1016/s0041-0101(98)00096-8

PMID:9690776

Abstract

Chironex fleckeri, the northern Australian box jellyfish produces one of, if not, the most potent animal venoms. Study of the venom has been hampered by the limits of the animals' range and the venom's thermolability. Using retained lethality and native polyacrylamide gel electrophoresis (NPAGE), we show that lyophilization of autolysis isolated nematocysts is an effective method of transporting the venom. In addition, Sephadex G-200 chromatography, spin concentration, and NPAGE fail to demonstrate the presence of a 600 kDa protein to which the bulk of the lethal activity has been ascribed. Sodium dodecyl sulfate capillary electrophoresis of crude venom yields several protein bands with a molecular weight range of 30-200 kDa. Freeze-thaw studies show a loss of activity and NPAGE bands after two freeze thaw cycles.

摘要

澳大利亚北部的箱形水母——费氏海蜇，其产生的毒液即便不是最具毒性的动物毒液之一，也是其中之一。毒液的研究因该动物分布范围的限制以及毒液的热不稳定性而受阻。通过保留致死性和天然聚丙烯酰胺凝胶电泳（NPAGE），我们表明冻干自溶分离的刺丝囊是一种运输毒液的有效方法。此外，葡聚糖G - 200色谱法、离心浓缩法和NPAGE均未能证明存在一种被认为具有大部分致死活性的600 kDa蛋白质。粗毒液的十二烷基硫酸钠毛细管电泳产生了几条分子量范围在30 - 200 kDa的蛋白带。冻融研究表明，经过两个冻融循环后，活性和NPAGE条带会丧失。