Matsushita H, Shibata Y, Fuse K, Kimura M, Iinuma K
Department of Pathology, Toranomon Hospital, Tokyo, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(4):237-9. doi: 10.1007/BF02896581.
We report a method of sex chromatin analysis of lymphocytes separated from host organs in transfusion-associated graft-versus-host disease (GVHD), which enabled the demonstration of invasion by donor lymphocytes. The lymphocytes examined were separated from deparaffinized tissue blocks of skin, spleen and bone marrow from two female patients with transfusion-associated GVHD by incubation in 0.5% pepsin. The tissues had been removed at autopsy and fixed in formalin. Sex chromatin analysis was performed by fluorescence microscopy on separated lymphocytes stained with 0.005% quinacrine dihydrochloride. By this means Y chromatin-positive (i.e. male) lymphocytes were demonstrated in the skin, spleen and bone marrow of both female patients.
我们报告了一种用于分析输血相关移植物抗宿主病（GVHD）中从宿主器官分离出的淋巴细胞的性染色质的方法，该方法能够证实供体淋巴细胞的侵袭。所检测的淋巴细胞是通过在0.5%胃蛋白酶中孵育，从两名患有输血相关GVHD的女性患者的皮肤、脾脏和骨髓的石蜡包埋组织块中分离出来的。这些组织在尸检时取出并固定于福尔马林中。通过荧光显微镜对用0.005%盐酸喹吖因染色的分离淋巴细胞进行性染色质分析。通过这种方法，在两名女性患者的皮肤、脾脏和骨髓中均证实了Y染色质阳性（即男性）淋巴细胞。
