Davis J E, Cook R G, Van M, Rich R R
Graduate School of Biomedical Sciences, University of Texas, Houston.
Immunogenetics. 1988;28(3):171-81. doi: 10.1007/BF00375856.
Mechanisms to account for the unusual properties of a DR1 alpha beta complex (designated DRgp50) that is resistant to dissociation under normal conditions utilized were investigated. Expression of this DRgp50 complex is highly correlated with the failure of cells from certain DR1 individuals (DR1x) to stimulate specific DR1-restricted or alloreactive T-cell clones. Pulse/chase experiments demonstrated that this DRgp50 complex was not detectable until approximately 1 h of chase. The DR1 alpha and beta chains associated into the heterodimer in the absence of glycosylation and alterations in the number of oligosaccharides or sialylation of cell surface forms were not evident when compared with normal DR1 alpha and beta chains. Restriction fragment length polymorphism patterns of DR beta genes from normal (DR1n) and DR1x individuals were indistinguishable. However, a difference in the alpha chain genes between DR1n and DR1x individuals was revealed using Bgl II. This Bgl II restriction site mapped to the 3' untranslated region of DR alpha and represents a new genomic marker to distinguish this functional and biochemical variant of DR1.
研究了在正常使用条件下对解离具有抗性的DR1αβ复合物（命名为DRgp50）异常特性的机制。这种DRgp50复合物的表达与某些DR1个体（DR1x）的细胞无法刺激特定的DR1限制性或同种异体反应性T细胞克隆高度相关。脉冲/追踪实验表明，直到追踪约1小时后才能检测到这种DRgp50复合物。与正常的DR1α和β链相比，在没有糖基化的情况下，DR1α和β链缔合成异二聚体，并且细胞表面形式的寡糖数量或唾液酸化的改变并不明显。正常（DR1n）和DR1x个体的DRβ基因的限制性片段长度多态性模式无法区分。然而，使用Bgl II揭示了DR1n和DR1x个体之间α链基因的差异。这个Bgl II限制性位点定位于DRα的3'非翻译区，代表了一种新的基因组标记，用于区分DR1的这种功能和生化变体。
