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从一个cDNA克隆推导的HLA - dRβ链的完整序列以及多个非等位DRβ链基因的鉴定。

Complete sequence of an HLA-dR beta chain deduced from a cDNA clone and identification of multiple non-allelic DR beta chain genes.

作者信息

Long E O, Wake C T, Gorski J, Mach B

机构信息

Department of Microbiology, University of Geneva Medical School, Switzerland.

出版信息

EMBO J. 1983;2(3):389-94. doi: 10.1002/j.1460-2075.1983.tb01435.x.

DOI:10.1002/j.1460-2075.1983.tb01435.x
PMID:11894954
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC555145/
Abstract

At least three polymorphic class II antigens are encoded in the human major histocompatibility complex (HLA): DR, DC and SB. cDNA clones encoding beta chains of HLA-DR antigen, derived from mRNA of a heterozygous B-cell line, were isolated and could be divided into four subsets, clearly distinct from cDNA clones encoding DC beta chains. Therefore, at least two non-allelic DR beta chain genes exist. The complete sequence of one of the DR beta chain cDNA clones is presented. It defines a putative signal sequence, two extracellular domains, a trans-membrane region and a cytoplasmic tail. Comparison with a DC beta chain cDNA clone revealed a homology of 70% between the two beta chains and that the two genes diverged under relatively little selective pressure. A set of amino acids conserved in immunoglobulin molecules was found to be identical in both DR and DC beta chains. Comparison of the DR beta chain sequence with the amino acid sequence of another DR beta chain revealed a homology of 87% and that most differences are single amino acid substitutions. Allelic polymorphism in DR beta chains has probably not arisen by changes in long blocks of sequence.

摘要

人类主要组织相容性复合体（HLA）中至少编码了三种多态性II类抗原：DR、DC和SB。从杂合B细胞系的mRNA中分离出编码HLA - DR抗原β链的cDNA克隆，这些克隆可分为四个亚组，与编码DCβ链的cDNA克隆明显不同。因此，至少存在两个非等位的DRβ链基因。本文给出了其中一个DRβ链cDNA克隆的完整序列。它定义了一个推定的信号序列、两个细胞外结构域、一个跨膜区域和一个胞质尾。与DCβ链cDNA克隆的比较显示，两条β链之间的同源性为70%，且这两个基因在相对较小的选择压力下发生了分化。发现在免疫球蛋白分子中保守的一组氨基酸在DR和DCβ链中是相同的。将DRβ链序列与另一条DRβ链的氨基酸序列进行比较，同源性为87%，且大多数差异是单个氨基酸替换。DRβ链中的等位基因多态性可能并非由长序列块的变化引起。

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