Araújo-Jorge T C, De Souza W
Departamento de Ultraestrutura e Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil.
Acta Trop. 1988 Jun;45(2):127-36.
The ability of the surface galactose (Gal)/N-acetyl-D-galactosamine (GalNAc) receptor of mouse peritoneal macrophages to recognize bloodstream trypomastigotes of Trypanosoma cruzi was examined. The parasite's uptake is improved by its desialylation and impaired by its treatment with Gal or GalNAc-binding lectins. Further incubation of asialoparasites with lectins for Gal-blockage (PNA and RCA I) reverses, in a dose-dependent way, 35-80% of the neuraminidase effect on the endocytosis of T. cruzi. Similar effects were observed when lectins for GalNAc-blockage (PHA, WPA and DBA) were used. Asialoerythrocytes or galactosyl-oligosaccharides added during the parasite-cell interaction assays, also competed with the normal or desialylated tryptomastigotes for receptors on the host cell surface, inhibiting their uptake and reversing the effect of neuraminidase. Although indirect, these results are strongly suggestive that the Gal/GalNAc recognition system of the macrophages is involved in the interiorization of T. cruzi.
对小鼠腹腔巨噬细胞表面半乳糖（Gal）/N-乙酰-D-半乳糖胺（GalNAc）受体识别克氏锥虫血流型锥鞭毛体的能力进行了检测。寄生虫经去唾液酸化后摄取能力增强，而用Gal或GalNAc结合凝集素处理则会损害其摄取能力。用凝集素（PNA和RCA I）对去唾液酸寄生虫进行进一步孵育以阻断Gal，可呈剂量依赖性地逆转神经氨酸酶对克氏锥虫内吞作用35%-80%的影响。使用针对GalNAc阻断的凝集素（PHA、WPA和DBA）时也观察到类似效果。在寄生虫与细胞相互作用试验期间添加去唾液酸红细胞或半乳糖基寡糖，也会与正常或去唾液酸化的锥鞭毛体竞争宿主细胞表面的受体，抑制其摄取并逆转神经氨酸酶的作用。尽管是间接的，但这些结果强烈提示巨噬细胞的Gal/GalNAc识别系统参与了克氏锥虫的内化过程。
