de Araújo Jorge T C, de Souza W
Acta Trop. 1984 Mar;41(1):17-28.
The effect of mild enzyme (trypsin, neuraminidase) treatment, periodate treatment and addition of carbohydrates (mono, di-, and polysaccharides) on the ingestion of Trypanosoma cruzi epimastigotes and trypomastigotes by mouse macrophages was studied. Trypsin treatment did not interfere with the ingestion of epimastigotes but did, however, increase the ingestion of trypomastigotes by mouse peritoneal macrophages. Neuraminidase and periodate treatment of the parasites increased the uptake of epi- and trypomastigote forms. The neuraminidase effect was partially blocked by galactose or N-acetylgalactosamine. Galactose, mannose, fucose, N-acetylglucosamine, and N-acetylgalactosamine had an influence on the ingestion of T. cruzi by macrophages. This effect was dependent on the strain of parasite tested, and the medium used to cultivate the epimastigotes. The results obtained, in conjunction with the work of others, suggest that glycoproteins and/or glycolipids on the parasite and/or macrophage surface are involved in the T. cruzi-macrophage interaction.
研究了温和酶（胰蛋白酶、神经氨酸酶）处理、高碘酸盐处理以及添加碳水化合物（单糖、二糖和多糖）对小鼠巨噬细胞摄取克氏锥虫前鞭毛体和锥鞭毛体的影响。胰蛋白酶处理不干扰前鞭毛体的摄取，但会增加小鼠腹腔巨噬细胞对锥鞭毛体的摄取。对寄生虫进行神经氨酸酶和高碘酸盐处理会增加前鞭毛体和锥鞭毛体形式的摄取。半乳糖或N-乙酰半乳糖胺可部分阻断神经氨酸酶的作用。半乳糖、甘露糖、岩藻糖、N-乙酰葡糖胺和N-乙酰半乳糖胺对巨噬细胞摄取克氏锥虫有影响。这种影响取决于所测试的寄生虫菌株以及用于培养前鞭毛体的培养基。所获得的结果与其他人的研究工作相结合，表明寄生虫和/或巨噬细胞表面的糖蛋白和/或糖脂参与了克氏锥虫与巨噬细胞的相互作用。
