He Q, Beyers A D, Barclay A N, Williams A F
MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, England.
Cell. 1988 Sep 23;54(7):979-84. doi: 10.1016/0092-8674(88)90112-2.
The CD2 antigen can mediate mitogenesis of T lymphocytes after binding combinations of monoclonal antibodies. To examine the importance of the cytoplasmic domain in signaling, rat CD2 cDNA has been transfected into the human Jurkat cell line and triggering of an increase in cytoplasmic free Ca2+ concentration [( Ca2+]i) has been assayed. In cells expressing full-length CD2, a clear signal was triggered with anti-CD2 monoclonal antibodies. In contrast, a barely detectable increase in [Ca2+]i occurred with mutant rat CD2 molecules that included only 6 or 40 amino acids of the full-length cytoplasmic domain of 116 residues. It thus appears that the CD2 cytoplasmic domain plays a role in the signaling event.
CD2抗原在与单克隆抗体结合后可介导T淋巴细胞的有丝分裂。为了研究胞质结构域在信号传导中的重要性，已将大鼠CD2 cDNA转染到人Jurkat细胞系中，并检测了细胞质游离Ca2+浓度([Ca2+]i)的增加。在表达全长CD2的细胞中，抗CD2单克隆抗体触发了清晰的信号。相比之下，仅包含116个残基的全长胞质结构域中6个或40个氨基酸的突变大鼠CD2分子引发的[Ca2+]i增加几乎检测不到。因此，CD2胞质结构域似乎在信号传导事件中发挥作用。
