Olianas M C, Onali P
Department of Neurosciences, University of Cagliari, Italy.
Eur J Pharmacol. 1988 Jun 10;150(3):389-92. doi: 10.1016/0014-2999(88)90025-8.
Corticotropin-releasing factor (CRF) caused a concentration-dependent increase in dopamine (DA) synthesis measured in rat striatal synaptosomes. The concentrations of CRF producing half-maximal and maximal stimulation were 40 and 300 nM, respectively. The maximal effect corresponded to a 34% increase from the control level of DA synthesis. CRF 1 microM increased DA synthesis in synaptosomes of mouse striatum by 48%. Pretreatment of striatal synaptosomes with CRF produced a persistent activation of tyrosine hydroxylase assayed in extracts of lysed synaptosomes. These results suggest that CRF may play a modulatory role in striatal DA synthesis by acting on receptors located on DA terminals.
促肾上腺皮质激素释放因子（CRF）可使大鼠纹状体突触体中测得的多巴胺（DA）合成呈浓度依赖性增加。产生半数最大刺激和最大刺激的CRF浓度分别为40和300 nM。最大效应相当于DA合成对照水平增加34%。1 microM的CRF使小鼠纹状体突触体中的DA合成增加48%。用CRF预处理纹状体突触体可使裂解的突触体提取物中检测到的酪氨酸羟化酶持续激活。这些结果表明，CRF可能通过作用于DA终末上的受体在纹状体DA合成中发挥调节作用。
