Karabatas L M, Arata M, Anaya L, Cresto J C, Pivetta O H, Basabe J C
Fundación Laboratorio de Investigaciones Pediátricas, Buenos Aires, Argentina.
Diabetologia. 1988 Jun;31(6):375-8. doi: 10.1007/BF02341506.
In a previous study in C57BL/KsJ mdb/mdb mice aged 4 to 12 days we observed a diminished first phase of glucose-induced insulin secretion in vitro, and alterations in the inhibitory effect of somatostatin on insulin secretion. This study explores, using perifused pancreatic slices, whether the reduced B-cell responsiveness to somatostatin in mdb/mdb mice can be overcome upon induction of a biphasic insulin release by using theophylline. Under these conditions our results show: (1) in mdb/mdb mice aged 4 to 6 days, the restoration of the first peak of insulin secretion overcomes the reduced B-cell sensitivity to somatostatin; and (2) in mdb/mdb mice aged 7 to 12 days, the addition of theophylline only causes a partial restoration of B-cell responsiveness to somatostatin, suggesting that other mechanisms could be involved in the progressive impairement of B-cell sensitivity to somatostatin inhibitory effect.
在先前一项针对4至12日龄C57BL/KsJ mdb/mdb小鼠的研究中，我们观察到体外葡萄糖诱导的胰岛素分泌的第一阶段有所减少，以及生长抑素对胰岛素分泌的抑制作用发生改变。本研究使用灌流胰腺切片，探究在通过茶碱诱导双相胰岛素释放时，mdb/mdb小鼠中B细胞对生长抑素反应性降低的情况是否能够得到克服。在这些条件下，我们的结果显示：（1）在4至6日龄的mdb/mdb小鼠中，胰岛素分泌第一个峰值的恢复克服了B细胞对生长抑素敏感性的降低；（2）在7至12日龄的mdb/mdb小鼠中，添加茶碱仅使B细胞对生长抑素的反应性部分恢复，这表明其他机制可能参与了B细胞对生长抑素抑制作用敏感性的逐渐受损。
