Barton S E, Maddox P H, Jenkins D, Edwards R, Cuzick J, Singer A
Department of Gynaecology, Whittington Hospital, London.
Lancet. 1988 Sep 17;2(8612):652-4. doi: 10.1016/s0140-6736(88)90469-2.
Langerhans' cells in cervical epithelium in colposcopic biopsy specimens were identified by immunocytochemical staining for S100 protein and T6 (CD1) antigen, and their density was quantified. Possible cofactors for the development of cervical neoplasia were examined for any effect on the cell counts per unit area. Current cigarette smoking was associated with a significant decrease in the Langerhans' cell population in both normal epithelium and lesions due to cervical intraepithelial neoplasia. Ex-smokers tended to have cell counts between those of smokers and non-smokers. There was a dose-response relation between number of cigarettes smoked daily and effect on cell counts. These findings of a local immunological effect of smoking on cervical epithelium may explain the means by which cigarette smoking contributes to the development of cervical neoplasia.
通过对S100蛋白和T6（CD1）抗原进行免疫细胞化学染色，鉴定阴道镜活检标本中宫颈上皮内的朗格汉斯细胞，并对其密度进行定量分析。研究了宫颈癌变发展的可能辅助因素对每单位面积细胞计数的任何影响。目前吸烟与正常上皮和宫颈上皮内瘤变所致病变中的朗格汉斯细胞数量显著减少有关。已戒烟者的细胞计数往往介于吸烟者和非吸烟者之间。每日吸烟量与对细胞计数的影响之间存在剂量反应关系。吸烟对宫颈上皮的这种局部免疫效应的这些发现，可能解释了吸烟导致宫颈癌变发展的机制。
