Falke N
Sektion Elektronenmikroskopie, Universität Ulm, F.R.G.
Neuropeptides. 1988 May-Jun;11(4):163-7. doi: 10.1016/0143-4179(88)90070-4.
The effects of the opioid peptide dynorphin (1-8) on oxytocin and vasopressin release at the level of isolated neurosecretory endings were investigated. Neurosecretory endings prepared by homogenization and centrifugation were placed on a filter and constantly superfused. Stimulated hormone release was evoked by potassium depolarization (30 mM) and simultaneous increase of the osmolarity (20 mosmol/1). Stimulation resulted in two peaks of hormone release--a short first peak and a longer second one. Addition of dynorphin (1-8) (10(-7) M) to the superfusion buffer significantly diminished the first peak of oxytocin release and totally abolished the second. There was no effect of dynorphin (1-8) on vasopressin release.
研究了阿片肽强啡肽（1-8）对离体神经分泌末梢水平催产素和血管升压素释放的影响。通过匀浆和离心制备的神经分泌末梢置于滤膜上并持续灌流。通过钾离子去极化（30 mM）和同时增加渗透压（20 mosmol/1）诱发激素释放。刺激导致激素释放出现两个峰值——一个短暂的第一个峰值和一个较长的第二个峰值。向灌流缓冲液中添加强啡肽（1-8）（10^-7 M）可显著减少催产素释放的第一个峰值，并完全消除第二个峰值。强啡肽（1-8）对血管升压素释放没有影响。
