通过在线透析法测定自由活动大鼠伏隔核中的多巴胺释放：阿扑吗啡和类神经安定肽去甲脑啡肽-γ-内啡肽的作用。

Dopamine release in the nucleus accumbens of freely moving rats determined by on-line dialysis: effects of apomorphine and the neuroleptic-like peptide desenkephalin-gamma-endorphin.

作者信息

Radhakishun F S, Westerink B H, van Ree J M

机构信息

Department of Medicinal Chemistry, State University Groningen, The Netherlands.

出版信息

Neurosci Lett. 1988 Jul 8;89(3):328-34. doi: 10.1016/0304-3940(88)90548-4.

DOI:10.1016/0304-3940(88)90548-4

PMID:2901702

Abstract

This study examined the effects of apomorphine, sulpiride, desenkephalin-gamma-endorphin (DE gamma E) and a combination of DE gamma E with apomorphine on the release of dopamine (DA) and its main metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the nucleus accumbens of freely moving rats. A fully automated on-line brain dialysis system was used. A small dose of s.c. administered apomorphine induced a decrease in the output of DA and DOPAC. Sulpiride, infused into the nucleus accumbens, induced a 2-fold increase in the output of DA, DOPAC and HVA. DE gamma E hardly modified either the basal release of DA, DOPAC and HVA or the apomorphine-induced attenuation of the release of DA and DOPAC. These results indicate a dissociation between the behavioural effects of DE gamma E and its effect on the release of DA in vivo.

摘要

本研究检测了阿扑吗啡、舒必利、去甲脑啡肽-γ-内啡肽（DEγE）以及DEγE与阿扑吗啡联用对自由活动大鼠伏隔核中多巴胺（DA）及其主要代谢产物3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）释放的影响。采用了全自动在线脑透析系统。皮下注射小剂量阿扑吗啡可使DA和DOPAC的输出量减少。向伏隔核内注入舒必利可使DA、DOPAC和HVA的输出量增加两倍。DEγE几乎不改变DA、DOPAC和HVA的基础释放量，也不改变阿扑吗啡诱导的DA和DOPAC释放的减弱。这些结果表明DEγE的行为效应与其对体内DA释放的影响之间存在分离。