Arakaki Paloma Rocha, Carvalho Fernanda Maria de, Castro Paulo Henrique Gomes de, Muniz José Augusto Pereira Carneiro, Valle Rodrigo Del Rio do
Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Folia Primatol (Basel). 2017;88(4):334-343. doi: 10.1159/000480501. Epub 2017 Oct 11.
Research on Neotropical primates' reproduction is necessary due to the lack of available information and the increasing threat to these species. Callimico goeldii is listed as Vulnerable on the IUCN Red List of Threatened Species. This study aimed to test rectal electrostimulation for semen collection and evaluate seminal characteristics. Therefore, semen from 6 captive Goeldi's monkeys was collected and, for the first time, seminal characteristics are described. Coagulum formation was noted in all ejaculates, and we obtained partial or complete liquefaction of the samples. Results were (means ± SD): volume = 26.9 ± 11.87 μL; pH = 7.61 ± 0.28; concentration = 143.18 ± 174.96 × 106 spermatozoa/mL; total sperm motility = 83.33 ± 5.16%; linear progressive motility = 46 ± 24.08%; plasma membrane integrity = 36.38 ± 16.11%; acrosome integrity using fast-green/bengal-rose staining = 63.41 ± 11.72%, and kit Spermac® = 69.36 ± 11.81%; abnormal sperm = 72.5 ± 17.7%, with 16.2 ± 7.7% major defects and 56.3 ± 10% minor defects; sperm with high mitochondrial activity class I = 16.45 ± 22.25%. Rectal electrostimulation was an efficient method for semen collection in this species. Investigations are required to improve semen collection and handling, including cryopreservation methods.
由于缺乏可用信息以及这些物种面临的威胁日益增加，对新热带灵长类动物繁殖的研究很有必要。白领伶猴在世界自然保护联盟濒危物种红色名录中被列为易危物种。本研究旨在测试直肠电刺激采精并评估精液特征。因此，采集了6只圈养白领伶猴的精液，并首次描述了精液特征。在所有射精样本中均观察到凝块形成，且样本实现了部分或完全液化。结果如下（均值±标准差）：体积 = 26.9 ± 11.87微升；pH值 = 7.61 ± 0.28；浓度 = 143.18 ± 174.96×10⁶精子/毫升；总精子活力 = 83.33 ± 5.16%；直线前进运动活力 = 46 ± 24.08%；质膜完整性 = 36.38 ± 16.11%；使用固绿/孟加拉玫瑰红染色法检测顶体完整性为63.41 ± 11.72%，使用Spermac®试剂盒检测为69.36 ± 11.81%；异常精子 = 72.5 ± 17.7%，其中主要缺陷占16.2 ± 7.7%，次要缺陷占56.3 ± 10%；线粒体活性高的I类精子 = 16.45 ± 22.25%。直肠电刺激是该物种采精的有效方法。需要开展研究以改进精液采集和处理方法，包括冷冻保存方法。
