Abdelhafidh Khazri, Mhadhbi Lazher, Mezni Ali, Badreddine Sellami, Beyrem Hamouda, Mahmoudi Ezzeddine
a Faculte des Sciences de Bizerte , Universite de Carthage , Bizerte , Tunisia.
Biomarkers. 2018 Mar;23(2):167-173. doi: 10.1080/1354750X.2017.1390609. Epub 2017 Oct 20.
Cypermethrin (CYP) is a synthetic pyrethroid insecticide used worldwide in agriculture, home pest control. The toxicity of CYP is well studied in many organisms.
The aim of present study was to investigate the protective effect of Zizyphus lotus (Zizyp) fruit against neurotoxicity and oxidative stress induced by CYP in mice.
Mice were divided into four groups of six each: groups I and II were used as control and CYP control (20 mg/kg body weight). While, groups III was orally treated with Zizyphus lotus fruit (5 g/kg body weight) plus CYP (20 mg/kg body weight) for 18 days. Furthermore, HPLC-ESI-MS-MS (Q-Tof) and GC-MS were used to identify the compounds fraction.
Antioxidant enzyme catalase (CAT), neurotoxicity enzyme acetylcholinesterase (AChE) activities and hydrogen peroxide (HO) malondialdehyde (MDA) levels were determined in the liver, kidney and heart. CYP caused decreased CAT activity, inhibition of AChE activity and increased the levels of HO and MDA in heart, liver and kidney.
Our results indicate that Zizyp fruit is markedly effective in protecting mice against CYP-induced biochemical changes. This protection may be due to its antioxidant property and scavenging ability against active free radicals.
氯氰菊酯（CYP）是一种合成拟除虫菊酯类杀虫剂，在全球范围内用于农业、家庭害虫防治。CYP的毒性在许多生物体中都有深入研究。
本研究旨在探讨荷叶（Zizyp）果实对CYP诱导的小鼠神经毒性和氧化应激的保护作用。
将小鼠分为四组，每组六只：第一组和第二组作为对照组和CYP对照组（20毫克/千克体重）。而第三组口服荷叶果实（5克/千克体重）加CYP（20毫克/千克体重），持续18天。此外，采用高效液相色谱-电喷雾串联质谱（Q-TOF）和气相色谱-质谱联用技术鉴定化合物组分。
测定了肝脏、肾脏和心脏中的抗氧化酶过氧化氢酶（CAT）、神经毒性酶乙酰胆碱酯酶（AChE）活性以及过氧化氢（HO）、丙二醛（MDA）水平。CYP导致心脏、肝脏和肾脏中CAT活性降低、AChE活性受到抑制，以及HO和MDA水平升高。
我们的结果表明，荷叶果实对保护小鼠免受CYP诱导的生化变化具有显著效果。这种保护作用可能归因于其抗氧化特性和对活性自由基的清除能力。
