Ji Xiaoyu, Liu Xiaoqiang, Peng Yuanxia, Zhan Ruoting, Xu Hui, Ge Xijin
Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, China.
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):318-324. doi: 10.1016/j.bbrc.2017.10.033. Epub 2017 Oct 7.
Emodin has a strong antibacterial activity, including methicillin-resistant Staphylococcus aureus (MRSA). However, the mechanism by which emodin induces growth inhibition against MRSA remains unclear. In this study, the isobaric tags for relative and absolute quantitation (iTRAQ) proteomics approach was used to investigate the modes of action of emodin on a MRSA isolate and methicillin-sensitive S. aureus ATCC29213(MSSA). Proteomic analysis showed that expression levels of 145 and 122 proteins were changed significantly in MRSA and MSSA, respectively, after emodin treatment. Comparative analysis of the functions of differentially expressed proteins between the two strains was performed via bioinformatics tools blast2go and STRING database. Proteins related to pyruvate pathway imbalance induction, protein synthesis inhibition, and DNA synthesis suppression were found in both methicillin-sensitive and resistant strains. Moreover, Interference proteins related to membrane damage mechanism were also observed in MRSA. Our findings indicate that emodin is a potential antibacterial agent targeting MRSA via multiple mechanisms.
大黄素具有很强的抗菌活性，包括对耐甲氧西林金黄色葡萄球菌（MRSA）。然而，大黄素诱导对MRSA生长抑制的机制仍不清楚。在本研究中，采用相对和绝对定量等压标签（iTRAQ）蛋白质组学方法来研究大黄素对一株MRSA分离株和甲氧西林敏感金黄色葡萄球菌ATCC29213（MSSA）的作用模式。蛋白质组学分析表明，大黄素处理后，MRSA和MSSA中分别有145和122种蛋白质的表达水平发生了显著变化。通过生物信息学工具blast2go和STRING数据库对两株菌株中差异表达蛋白质的功能进行了比较分析。在甲氧西林敏感和耐药菌株中均发现了与丙酮酸途径失衡诱导、蛋白质合成抑制和DNA合成抑制相关的蛋白质。此外，在MRSA中还观察到了与膜损伤机制相关的干扰蛋白。我们的研究结果表明，大黄素是一种通过多种机制靶向MRSA的潜在抗菌剂。
