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定量蛋白质组学分析揭示了牦牛睾丸发育过程中的关键蛋白。

Quantitative Proteomic Analysis Reveals Key Proteins Involved in Testicular Development of Yaks.

机构信息

Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China.

Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.

出版信息

Int J Mol Sci. 2024 Aug 2;25(15):8433. doi: 10.3390/ijms25158433.

DOI:10.3390/ijms25158433
PMID:39126002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11313431/
Abstract

Male reproductive health is largely determined already in the early development of the testis. Although much work has been carried out to study the mechanisms of testicular development and spermatogenesis, there was previously no information on the differences in the protein composition of yak testicles during early development. In this study, the protein profiles in the testicles of 6- (M6), 18- (M18), and 30-month-old (M30) yaks were comparatively analyzed using TMT proteomics. A total of 5521 proteins were identified, with 13, 1295, and 1397 differentially expressed proteins (DEPs) in 30- vs. 18-, 18- vs. 6-, and 30- vs. 6-month-old testes, respectively. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEPs were mainly involved in signaling pathways related to testicular development and spermatogenesis, including the MAPK, PI3K-Akt, Wnt, mTOR, TGF-β, and AMPK signaling pathways. Furthermore, we also identified eight potential proteins (TEX101, PDCL2, SYCP2, SYCP3, COL1A1, COL1A2, ADAM10, and ATF1) that may be related to the testicular development and spermatogenesis of yaks. This study may provide new insights into the molecular mechanisms of the testicular development and spermatogenesis of yaks.

摘要

雄性生殖健康在很大程度上取决于睾丸的早期发育。尽管已经进行了大量的研究来探索睾丸发育和精子发生的机制，但之前没有关于牦牛睾丸在早期发育过程中蛋白质组成差异的信息。在这项研究中，我们使用 TMT 蛋白质组学比较分析了 6 月龄（M6）、18 月龄（M18）和 30 月龄（M30）牦牛睾丸中的蛋白质图谱。共鉴定出 5521 种蛋白质，30 月龄与 18 月龄、18 月龄与 6 月龄、30 月龄与 6 月龄睾丸相比，分别有 13、1295 和 1397 个差异表达蛋白（DEPs）。GO 注释和 KEGG 富集分析表明，DEPs 主要参与与睾丸发育和精子发生相关的信号通路，包括 MAPK、PI3K-Akt、Wnt、mTOR、TGF-β和 AMPK 信号通路。此外，我们还鉴定了 8 个可能与牦牛睾丸发育和精子发生相关的潜在蛋白（TEX101、PDCL2、SYCP2、SYCP3、COL1A1、COL1A2、ADAM10 和 ATF1）。本研究可为牦牛睾丸发育和精子发生的分子机制提供新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/f807947cfd0c/ijms-25-08433-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/eaba11f38a2c/ijms-25-08433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/07f2b9874b31/ijms-25-08433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/f807947cfd0c/ijms-25-08433-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/eaba11f38a2c/ijms-25-08433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/07f2b9874b31/ijms-25-08433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e5/11313431/f807947cfd0c/ijms-25-08433-g003.jpg

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