Wang Hong, Zheng Han, Li Qun, Xu Yanmei, Wang Jianping, Du Pengcheng, Li Xinqiong, Liu Xiang, Zhang Ling, Zou Nianli, Yan Guodong, Zhang Zhengdong, Jing Huaiqi, Xu Jianguo, Xiong Yanwen
Zigong Center for Disease Control and Prevention, Zigong, China.
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Front Microbiol. 2017 Sep 26;8:1857. doi: 10.3389/fmicb.2017.01857. eCollection 2017.
is a newly described and emerging diarrheagenic pathogen responsible for outbreaks of gastroenteritis. Serotyping plays an important role in diagnosis and epidemiological studies for pathogens of public health importance. The diversity of O-antigen biosynthesis gene clusters (O-AGCs) provides the primary basis for serotyping. However, little is known about the distribution and diversity of O-AGCs of strains. Here, we presented a complete sequence set for the O-AGCs from 52 strains and identified seven distinct O-AGCs. Six of these were also found in 15 genomes of strains deposited in the public database. Possession of / genes in each O-AGC strongly suggest that O-antigens of were synthesized by the Wzx/Wzy-dependent pathway. Furthermore, we performed an O-antigen serotyping scheme for based on specific antisera against seven O-antigens and a high throughput xTAG Luminex assay to simultaneously detect seven O-AGCs. Both methods accurately identified serotypes of 64 tested strains. Our data revealed the high-level diversity of O-AGCs in . We also provide valuable methods to reliably identify and serotype this bacterium.
是一种新描述的、正在出现的致腹泻病原体，可导致肠胃炎暴发。血清分型在对具有公共卫生重要性的病原体的诊断和流行病学研究中发挥着重要作用。O抗原生物合成基因簇（O-AGCs）的多样性为血清分型提供了主要依据。然而，关于菌株O-AGCs的分布和多样性知之甚少。在此，我们展示了52株菌株O-AGCs的完整序列集，并鉴定出7种不同的O-AGCs。其中6种也在公共数据库中保存的15个菌株基因组中被发现。每个O-AGC中/基因的存在强烈表明，的O抗原是通过Wzx/Wzy依赖性途径合成的。此外，我们基于针对7种O抗原的特异性抗血清和高通量xTAG Luminex检测法，为进行了O抗原血清分型方案，以同时检测7种O-AGCs。两种方法都准确鉴定了64株受试菌株的血清型。我们的数据揭示了中O-AGCs的高度多样性。我们还提供了可靠鉴定该细菌及其血清型的有价值方法。
