Li Xinjuan, Wang Kai, Ma Nana, Jia Xianbin
Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, State Education Ministry of China, Henan Normal University, Xinxiang, China.
Front Chem. 2017 Sep 26;5:69. doi: 10.3389/fchem.2017.00069. eCollection 2017.
Naked-eye detection of amino acids (AA) in water is of great significance in the field of bioanalytical applications. Herein, polymerized ionic liquids (PILs) with controlled chain length structures were synthesized via reversible addition-fragmentation chain-transfer (RAFT) polymerization and post-quaternization approach. The AA recognition performance of PILs with different alkyl chain lengths and molecular weights was evaluated by naked-eye color change and ultraviolet-visible (UV-vis) spectral studies. These PILs were successfully used for highly sensitive and selective detection of Arg, Lys, and His in water. The recognition performance was improved effectively with increased molecular weight of PILs. The biosensitivity of the PILs in water was strongly dependent on their aggregation effect and polarization effect. Highly sensitive and selective detection of AA was successfully accomplished by introducing positively charged pyridinium moieties and controlled RAFT radical polymerization.
水中氨基酸（AA）的裸眼检测在生物分析应用领域具有重要意义。在此，通过可逆加成-断裂链转移（RAFT）聚合和后季铵化方法合成了具有可控链长结构的聚合离子液体（PILs）。通过裸眼颜色变化和紫外-可见（UV-vis）光谱研究评估了不同烷基链长度和分子量的PILs对AA的识别性能。这些PILs成功用于水中精氨酸（Arg）、赖氨酸（Lys）和组氨酸（His）的高灵敏和选择性检测。随着PILs分子量的增加，识别性能得到有效提高。PILs在水中的生物敏感性强烈依赖于它们的聚集效应和极化效应。通过引入带正电荷的吡啶鎓部分和可控的RAFT自由基聚合，成功实现了对AA的高灵敏和选择性检测。
