Liu Boshi, Huang Renliang, Yu Yanjun, Su Rongxin, Qi Wei, He Zhimin
State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
School of Environmental Science and Engineering, Tianjin University, Tianjin, China.
Front Chem. 2018 Apr 4;6:94. doi: 10.3389/fchem.2018.00094. eCollection 2018.
Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of and , and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a "double calibration curve" method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths.
赭曲霉毒素A（OTA）是由 和 的代谢产生的一种霉菌毒素，对人类、家畜和家禽具有极高的毒性。然而，传统的OTA检测方法昂贵且复杂。除了OTA适体之外，高浓度的OTA本身也能吸附在金纳米颗粒（AuNPs）表面，并进一步抑制AuNPs盐聚集。我们在此报告一种利用AuNPs及其聚集体的局域表面等离子体共振效应的新型OTA检测方法。仅从一条单一的线性校准曲线获得的结果不可靠，因此我们开发了一种“双校准曲线”方法来解决这一问题并拓宽OTA检测范围。我们还检测了许多其他分析物，并进一步讨论了与AuNPs结合的分析物的结构特性。我们发现，由于基于AuNP聚集的方法中各种分析物的结合强度不同，在检测这些分析物时必须考虑各种因素。
