Gordon M Y
Leukaemia Research Fund Centre, Institute of Cancer Research, London, UK.
Bone Marrow Transplant. 1988 Jul;3(4):247-51.
The source of stromal cells following bone marrow transplantation has been investigated by culturing marrow cells obtained from patients who have a graft cells from a donor of the opposite sex. Two culture systems have been used for these studies. The first supports the proliferation of fibroblast colony-forming cells (F-CFC) and the formation of confluent layers of fibroblastoid cells; the second (the long-term bone marrow culture system) results in the formation of a complex and heterogeneous layer of adherent cells composed of fibroblasts, fat cells, endothelial cells, macrophages and 'blanket' cells. A survey of the work done using these culture systems shows that, although the issue of the transplantability of stromal cells remains controversial, it now seems reasonably certain that repopulation of the fibroblast component by donor cells is rare. In contrast, one study has shown that endothelial-like cells can engraft in transplant recipients. Another study has demonstrated a donor origin for the macrophages found in long-term cultures of marrow from transplanted patients. This finding might have been predicted because macrophages are derived from haemopoietic stem cells.
通过培养从接受异性供体移植细胞的患者身上获取的骨髓细胞，对骨髓移植后基质细胞的来源进行了研究。这些研究使用了两种培养系统。第一种支持成纤维细胞集落形成细胞（F-CFC）的增殖以及成纤维样细胞汇合层的形成；第二种（长期骨髓培养系统）会导致形成由成纤维细胞、脂肪细胞、内皮细胞、巨噬细胞和“覆盖”细胞组成的复杂且异质的贴壁细胞层。对使用这些培养系统所做工作的调查表明，尽管基质细胞的可移植性问题仍存在争议，但现在似乎有理由确定供体细胞对成纤维细胞成分的重新填充很少见。相比之下，一项研究表明内皮样细胞可以在移植受者体内植入。另一项研究证明了在移植患者骨髓的长期培养物中发现的巨噬细胞具有供体来源。这一发现可能是可以预见的，因为巨噬细胞源自造血干细胞。
