Lennon J E, Micklem H S
Exp Hematol. 1986 May;14(4):287-92.
Adherent layers from hematopoietically active long-term bone marrow cultures (LTBMC), incubated with fluorescent beads, were analyzed for autofluorescence and phagocytic ability, using a fluorescence-activated cell sorter (FACS). Four groups of cells were separated from the adherent layers, including a group of large polygonal fibroblastoid stromal cells. Long-term chimeras were made by lethal irradiation of CBA/Ca (CBA) and C57Bl6/J (B6) mice and repopulation with phosphoglycerate kinase (PGK-1) alloenzyme-congenic bone marrow cells. Hematopoietically active LTBMC were established from such chimeras, and donor and host contributions of FACS-sorted adherent-layer cells were measured. While macrophages and other hematopoietic cells were of donor origin, the fibroblastoid stromal cells were mainly or entirely host derived.
将来自造血活跃的长期骨髓培养物（LTBMC）的贴壁层与荧光珠一起孵育，使用荧光激活细胞分选仪（FACS）分析其自发荧光和吞噬能力。从贴壁层中分离出四组细胞，其中包括一组大的多边形成纤维细胞样基质细胞。通过对CBA/Ca（CBA）和C57Bl6/J（B6）小鼠进行致死性照射并用磷酸甘油酸激酶（PGK-1）同种酶同基因骨髓细胞进行再填充，制备长期嵌合体。从这些嵌合体中建立造血活跃的LTBMC，并测量FACS分选的贴壁层细胞的供体和宿主贡献。虽然巨噬细胞和其他造血细胞来源于供体，但成纤维细胞样基质细胞主要或完全来源于宿主。
