Schäck Luisa Marilena, Clausen Jan-Dierk, Noack Sandra, Harb Afif, Krettek Christian, Neunaber Claudia
Unfallchirurgie, Experimentelle Unfallchirurgie - Labor für Muskulo-Skeletales Trauma & Regenerative Therapien, Medizinische Hochschule Hannover, Klinik für Unfallchirurgie, Carl-Neuberg-Str. 1, OE 6230, 30625, Hannover, Deutschland.
Unfallchirurg. 2017 Nov;120(11):918-926. doi: 10.1007/s00113-017-0418-y.
The clinical outcome of fresh allogeneic osteochondral allografts (OCA) is greatly dependent on the number of viable chondrocytes at the time of implantation. The selection and preparation of a suitable recipient can be very time-consuming and the number of tissue donors is greatly limited; therefore, the preservation of high allograft viability before transplantation is a focal point of current research.
The objective of this review is to give an overview of established storage strategies for OCA and to serve as a decision-making aid for German clinics in the choice of a suitable storage strategy.
A search of the literature published between January 2002 and May 2017 was independently performed by two persons with respect to original works on storage strategies of OCA with a focus on storage medium, use of fetal bovine serum, storage temperature and change of medium. A total of 20 suitable studies were selected for this review.
Based on the current studies a clearly superior storage solution could not be identified; however, storage at 4 °C seems to give better results with respect to cell viability than storage at 37 °C. High chondrocyte viability rates after 28 days of storage were also achieved using media without the addition of fetal bovine serum.
A major difficulty in comparing the relevant studies on storage solutions is that multiple aspects in the study design varied between the studies. Due to this no definite conclusion on what the ideal storage strategy should look like could be drawn. Further studies are needed to conclusively show whether cell culture medium-based storage solutions are truly superior to those based on Ringer-lactate solutions.
新鲜同种异体骨软骨移植（OCA）的临床结果在很大程度上取决于植入时存活软骨细胞的数量。合适受体的选择和准备可能非常耗时，并且组织供体数量极为有限；因此，移植前保持同种异体移植物的高存活率是当前研究的重点。
本综述的目的是概述已确立的OCA储存策略，并为德国诊所选择合适的储存策略提供决策辅助。
两名研究人员独立检索了2002年1月至2017年5月发表的关于OCA储存策略的原始文献，重点关注储存介质、胎牛血清的使用、储存温度和培养基更换。本综述共选择了20项合适的研究。
基于目前的研究，无法确定一种明显更优的储存溶液；然而，在细胞活力方面，4℃储存似乎比37℃储存效果更好。使用不添加胎牛血清的培养基在储存28天后也能实现较高的软骨细胞存活率。
比较有关储存溶液的相关研究的一个主要困难在于，研究设计中的多个方面在不同研究之间存在差异。因此，无法就理想的储存策略应该是什么样得出明确结论。需要进一步的研究来最终表明基于细胞培养基的储存溶液是否真的优于基于乳酸林格氏液的储存溶液。
