Entomological Research Institute, Ayub Agricultural Research Institute|, Faisalabad, Pakistan.
Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China.
Environ Sci Pollut Res Int. 2017 Dec;24(36):28165-28174. doi: 10.1007/s11356-017-0383-6. Epub 2017 Oct 10.
The stored grain insects cause great damage to grains under storage conditions. Synthetic insecticides and fumigants are considered as key measures to control these stored grain insect pests. However, the major issue with these chemicals is grain contamination with chemical residues and development of resistance by insect pests to these chemicals. Biological control is considered as a potential alternative to chemical control especially with the use of pathogens, alone or in combination with selective insecticides. The present study was conducted to evaluate the synergism of Metarhizium anisopliae with diatomaceous earth (DE) and thiamethoxam against four insect pests on the stored wheat grains. In the first bioassay, the M. anisopliae was applied at 1.4 × 10 and 1.4 × 10conidia/ml alone and in integration with two concentrations (250 and 500 ppm) of tested DE. The tested fungus when combined with DE and thiamethoxam possessed synergistic impact as compared to their individual efficacy. Adult mortality increased with respect to increased exposure interval and doses. In the second bioassay, M. anisopliae was applied at 1.4 × 10 conidia/ml individually and in combination with three concentrations (0.50, 0.75, and 1.00 ppm) of thiamethoxam. Results concluded that M. anisopliae integrated with DE and thiamethoxam provides more effective control of stored grain insect pests.
储粮害虫在储存条件下会对粮食造成严重损害。合成杀虫剂和熏蒸剂被认为是控制这些储粮害虫的关键措施。然而，这些化学物质的主要问题是化学残留物对粮食的污染以及害虫对这些化学物质产生抗药性。生物防治被认为是化学防治的一种潜在替代品，特别是在使用病原体时，单独使用或与选择性杀虫剂结合使用。本研究旨在评估绿僵菌与硅藻土（DE）和噻虫嗪对四种储粮害虫的协同作用。在第一个生物测定中，绿僵菌以 1.4×10 和 1.4×10 个分生孢子/ml 的浓度单独应用，并与两种浓度（250 和 500 ppm）的测试 DE 相结合。与单独使用相比，测试真菌与 DE 和噻虫嗪结合具有协同作用。成虫死亡率随着暴露时间和剂量的增加而增加。在第二个生物测定中，绿僵菌以 1.4×10 个分生孢子/ml 的浓度单独应用，并与噻虫嗪的三种浓度（0.50、0.75 和 1.00 ppm）相结合。结果表明，绿僵菌与 DE 和噻虫嗪结合使用可以更有效地控制储粮害虫。
