Nynca Joanna, Dietrich Mariola A, Ciereszko Andrzej
Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
Methods Mol Biol. 2018;1664:203-219. doi: 10.1007/978-1-4939-7268-5_16.
Two-dimensional difference gel electrophoresis (2D-DIGE) appears to be especially useful in quantitative approaches, allowing the co-separation of proteins of control samples from proteins of treatment/disease samples on the same gel, eliminating gel-to-gel variability. The principle of 2D-DIGE is to label proteins prior to isoelectric focusing and use three spectrally resolvable fluorescent dyes, allowing the independent labeling of control and experimental samples. This procedure makes it possible to reduce the number of gels in an experiment, allowing the accurate and reproducible quantification of multiple samples. 2D-DIGE has been found to be an excellent methodical tool in several areas of fish research, including environmental pollution and toxicology, the mechanisms of development and disorders, reproduction, nutrition, evolution, and ecology.
二维差异凝胶电泳（2D-DIGE）在定量分析中似乎特别有用，它能在同一凝胶上共同分离对照样品的蛋白质和处理/疾病样品的蛋白质，消除凝胶间的差异。2D-DIGE的原理是在等电聚焦之前对蛋白质进行标记，并使用三种光谱可分辨的荧光染料，从而实现对对照样品和实验样品的独立标记。该方法能够减少实验中凝胶的使用数量，实现对多个样品的准确且可重复的定量分析。二维差异凝胶电泳已被证明是鱼类研究多个领域中出色的方法学工具，包括环境污染与毒理学、发育与疾病机制、繁殖、营养、进化和生态学等领域。
