Com Emmanuelle, Gruhler Albrecht, Courcol Martine, Gautier Jean-Charles
Disposition, Safety and Animal Research, sanofi-aventis R&D, Vitry-sur-Seine, France.
Methods Mol Biol. 2011;691:187-203. doi: 10.1007/978-1-60761-849-2_11.
In recent years, several global omics technologies have been increasingly used to better understand the molecular mechanisms of drug toxicity. Two-dimensional difference gel electrophoresis (2D-DIGE) is a large-scale proteomics high-resolution gel-based quantitative method widely used to detect protein expression alterations after drug treatment. The 2D-DIGE technology is based on the labeling of proteins with different fluorescent dyes, allowing the separation of different samples on the same gel with the use of an internal standard, thus reducing the complexity of spot pattern comparison and providing a reliable method applied to toxicology studies for the detection of modulated proteins in targeted organs.
近年来，几种全球组学技术越来越多地被用于更好地理解药物毒性的分子机制。二维差异凝胶电泳（2D-DIGE）是一种大规模蛋白质组学的基于凝胶的高分辨率定量方法，广泛用于检测药物处理后蛋白质表达的变化。2D-DIGE技术基于用不同荧光染料标记蛋白质，允许在使用内标的情况下在同一凝胶上分离不同样品，从而降低斑点模式比较的复杂性，并提供一种可靠的方法应用于毒理学研究，以检测靶器官中被调节的蛋白质。
