Zhang Wenzheng, Tang Jijun, Li Bingzhi, Yuan Yingjin
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China.
SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Sheng Wu Gong Cheng Xue Bao. 2016 Jul 25;32(7):901-911. doi: 10.13345/j.cjb.150442.
Chromosomal integration of heterologous genes or pathways is preferred over the use of episomal plasmids for its inherently stability and thus more desirable in the industrial setting. However, the position of integration of heterologous genes in the genome influences the expression levels. In combination of high throughput transformation of the Yeast Knock-out Collection (YKO) and FACS analysis, the position effect on heterologous reporter gene gfp was identified across the whole genome in yeast. In total 428 high-expressed sites and 444 low-expressed sites were spotted, providing massive data to analyze patterns and reasons for region dependency of gene expression on the genome-wide scale.
与使用附加体质粒相比，异源基因或途径的染色体整合因其固有的稳定性而更受青睐，因此在工业环境中更具优势。然而，异源基因在基因组中的整合位置会影响表达水平。结合酵母基因敲除文库（YKO）的高通量转化和荧光激活细胞分选（FACS）分析，在酵母全基因组中确定了异源报告基因gfp的位置效应。总共发现了428个高表达位点和444个低表达位点，为在全基因组范围内分析基因表达的区域依赖性模式和原因提供了大量数据。
