Havilio Moshe, Levanon Erez Y, Lerman Galia, Kupiec Martin, Eisenberg Eli
Compugen Ltd., 72 Pinchas Rosen St., Tel-Aviv 69512, Israel.
BMC Genomics. 2005 Jun 16;6:93. doi: 10.1186/1471-2164-6-93.
Recent studies in a growing number of organisms have yielded accumulating evidence that a significant portion of the non-coding region in the genome is transcribed. We address this issue in the yeast Saccharomyces cerevisiae.
Taking into account the absence of a significantly large yeast EST database, we use microarray expression data collected for genomic regions erroneously believed to be coding to study the expression pattern of non-coding regions in the Saccharomyces cerevisiae genome. We find that at least 164 out of 589 (28%) such regions are expressed under specific biological conditions. In particular, looking at the probes that are located opposing other known genes at the same genomic locus, we find that 88 out of 341 (26%) of these genes support antisense transcription. The expression patterns of these antisense genes are positively correlated. We validate these results using RT-PCR on a sample of 6 non-coding transcripts.
最近在越来越多生物体中的研究积累了越来越多的证据，表明基因组中很大一部分非编码区域被转录。我们在酿酒酵母中研究了这个问题。
考虑到缺乏大量的酵母EST数据库，我们使用针对错误地认为是编码区的基因组区域收集的微阵列表达数据，来研究酿酒酵母基因组中非编码区域的表达模式。我们发现，在589个这样的区域中，至少有164个（28%）在特定生物学条件下表达。特别是，查看位于同一基因组位点上与其他已知基因相对的探针，我们发现这些基因中有341个（26%）中的88个支持反义转录。这些反义基因的表达模式呈正相关。我们使用RT-PCR对6个非编码转录本的样本验证了这些结果。
