Pan Xiaohong, Xu Zhangyan, Li Lan, Shao Enshi, Chen Saili, Huang Tengzhou, Chen Zhi, Rao Wenhua, Huang Tianpei, Zhang Lingling, Wu Songqing, Guan Xiong
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Lab of Biopesticide and Chemical Biology, Ministry of Education & College of Plant Protection & College of Resources and Environmental Sciences & College of Life Sciences & Forestry College, Fujian Agriculture and Forestry University , Fuzhou, Fujian 350002, P. R. China.
Fujian-Taiwan Joint Center for Ecological Control of Crop Pests , Fuzhou, Fujian 350002, P. R. China.
J Agric Food Chem. 2017 Nov 1;65(43):9428-9434. doi: 10.1021/acs.jafc.7b03410. Epub 2017 Oct 23.
The traditional Bacillus thuringiensis (Bt) formulations for field applications are not resistant to harsh environmental conditions. Hence, the active ingredients of the Bt bioinsecticides could degrade quickly and has low anti-ultraviolet ability in the field, which significantly limits its practical application. In the present study, we developed an efficient and stable delivery system for Bt Cry11Aa toxins. We coated Cry11Aa proteins with Mg(OH) nanoparticles (MHNPs), and then assessed the effects of MHNPs on bioactivity and anti-ultraviolet ability of the Cry11Aa proteins. Our results indicated that MHNPs, like "coating clothes", could effectively protect the Cry protein and enhance the insecticidal bioactivity after UV radiation (the degradation rate was decreased from 64.29% to 16.67%). In addtion, MHNPs could improve the proteolysis of Cry11Aa in the midgut and aggravate the damage of the Cry protein to the gut epithelial cells, leading to increased insecticidal activity against Culex quinquefasciatus. Our results revealed that MHNPs, as an excellent nanocarrier, could substantially improve the insecticidal bioactivity and anti-ultraviolet ability of Cry11Aa.
传统的用于田间应用的苏云金芽孢杆菌（Bt）制剂不耐受恶劣的环境条件。因此，Bt生物杀虫剂的活性成分在田间可能会迅速降解，且抗紫外线能力较低，这显著限制了其实际应用。在本研究中，我们开发了一种高效且稳定的Bt Cry11Aa毒素递送系统。我们用氢氧化镁纳米颗粒（MHNPs）包裹Cry11Aa蛋白，然后评估MHNPs对Cry11Aa蛋白生物活性和抗紫外线能力的影响。我们的结果表明，MHNPs就像“给衣服涂层”一样，能够有效保护Cry蛋白，并在紫外线辐射后增强杀虫生物活性（降解率从64.29%降至16.67%）。此外，MHNPs可以提高Cry11Aa在中肠中的蛋白水解作用，并加剧Cry蛋白对肠道上皮细胞的损伤，从而提高对致倦库蚊的杀虫活性。我们的结果表明，MHNPs作为一种优异的纳米载体，可以大幅提高Cry11Aa的杀虫生物活性和抗紫外线能力。
