State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection & Key Lab of Biopesticide and Chemical Biology, Ministry of Education , Fujian Agriculture and Forestry University , Fuzhou , Fujian 350002 , People's Republic of China.
J Agric Food Chem. 2018 Apr 11;66(14):3651-3657. doi: 10.1021/acs.jafc.8b00575. Epub 2018 Apr 2.
Bacillus thuringiensis (Bt) can produce Cry proteins during the sporulation phase, and Cry protein is effective against lepidopteran, coleopteran, and dipteran insects and nematodes. However, Cry protein tends to be discharged into soil and nontarget plants through rainwater runoff, leading to reduced effective period toward target insects. In the present study, nano-Mg(OH) (magnesium hydroxide nanoparticles, MHNPs) were synthesized to control the loss of Cry1Ac protein and deliver protein to Helicoverpa armigera (Lepidoptera: Noctuidae). The results showed that Cry1Ac protein could be loaded onto MHNPs through electrostatic adsorption, and both MHNPs and Cry protein were stable during the adsorption process. Meanwhile, the Cry1Ac-loaded MHNPs could remain on the surface of cotton leaves, resulting in enhanced adhesion of Cry1Ac protein by 59.50% and increased pest mortality by 75.00%. Additionally, MHNPs could be slowly decomposed by acid medium and MHNPs showed no obvious influence on cotton, Bt, Escherichia coli, and H. armigera. Therefore, MHNPs could serve as an efficient nanocarrier for delivery of Cry1Ac protein and be used as a potential adjuvant for biopesticide in agricultural applications.
苏云金芽孢杆菌（Bt）在孢子形成阶段可以产生 Cry 蛋白，Cry 蛋白对鳞翅目、鞘翅目和双翅目昆虫和线虫有效。然而，Cry 蛋白往往会通过雨水径流排放到土壤和非靶标植物中，从而降低对靶标昆虫的有效期限。在本研究中，合成了纳米-Mg(OH)（氢氧化镁纳米粒子，MHNPs）来控制 Cry1Ac 蛋白的损失并将蛋白递送到棉铃虫（鳞翅目：夜蛾科）。结果表明，Cry1Ac 蛋白可以通过静电吸附负载到 MHNPs 上，并且在吸附过程中 MHNPs 和 Cry 蛋白都很稳定。同时，负载 Cry1Ac 的 MHNPs 可以留在棉花叶片表面，使 Cry1Ac 蛋白的粘附性提高 59.50%，害虫死亡率提高 75.00%。此外，MHNPs 可以被酸性介质缓慢分解，并且 MHNPs 对棉花、Bt、大肠杆菌和棉铃虫没有明显影响。因此，MHNPs 可以作为 Cry1Ac 蛋白传递的高效纳米载体，并可用作农业应用中生物农药的潜在佐剂。
