Willard-Gallo K E, Houck D W, Loken M R
International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Eur J Immunol. 1988 Sep;18(9):1453-61. doi: 10.1002/eji.1830180923.
This study provides new knowledge on the changes in protein expression that differentiate the functionally and phenotypically different cells of the human immune system. Purification by flow cytometry of normal lymphocytes (both T and B cells), monocytes and granulocytes, combined with high-resolution two-dimensional polyacrylamide gel electrophoresis, revealed reproducible qualitative and quantitative changes between these cell populations. Characteristic profiles of marker proteins for each cell type were identified. Determination of markers for T lymphocyte subpopulations was achieved by the comparative analysis of normal T cells separated on the basis of CD4 and CD8 expression in combination with the analysis of cells from patients with T cell chronic lymphocyte leukemia. These results suggest that the modulation or regulation of proteins is very strictly controlled in lymphoid differentiation, and that several quantitative and a few qualitative differences can give rise to completely different phenotypes. Thus, instead of detecting numerous random differences among lymphocyte protein patterns, rather stringent regulation of protein expression in each subpopulation was found.
本研究提供了有关蛋白质表达变化的新知识，这些变化区分了人类免疫系统中功能和表型不同的细胞。通过流式细胞术纯化正常淋巴细胞（T细胞和B细胞）、单核细胞和粒细胞，并结合高分辨率二维聚丙烯酰胺凝胶电泳，揭示了这些细胞群体之间可重复的定性和定量变化。确定了每种细胞类型的标记蛋白特征图谱。通过对基于CD4和CD8表达分离的正常T细胞进行比较分析，并结合对T细胞慢性淋巴细胞白血病患者细胞的分析，实现了T淋巴细胞亚群标记物的测定。这些结果表明，蛋白质的调节或调控在淋巴细胞分化过程中受到非常严格的控制，并且一些定量和少量定性差异可导致完全不同的表型。因此，并未检测到淋巴细胞蛋白质模式之间的众多随机差异，而是发现每个亚群中蛋白质表达受到相当严格的调控。
