Sanders M E, Makgoba M W, Sharrow S O, Stephany D, Springer T A, Young H A, Shaw S
Immunology Branch, National Cancer Institute, Bethesda, MD 20892.
J Immunol. 1988 Mar 1;140(5):1401-7.
Studies of cell-surface molecules involved in human T cell interaction reveal that differential expression of each of three adhesion molecules (LFA-3, CD2, and LFA-1) subdivides human peripheral blood T cells into major subpopulations. Systematic analysis of the relationship between expression of these and other markers of T cell subsets demonstrates a single major subset of human peripheral blood T lymphocytes distinguished by enhanced expression of LFA-3, CD2, LFA-1, and three other markers (CDw29 [4B4], UCHL1, and Pgp-1). Large differences in relative expression are observed for UCHL1 (29-fold) and LFA-3 (greater than 8-fold), and smaller differences (2- to 4-fold) are seen for CDw29, CD2, LFA-1, and Pgp-1. Bimodal distribution of LFA-3 is found on both CD4+ cells and on CD8+ cells as well as on B lymphocytes (CD19+). Neonatal T cells (CD3+) are comprised almost exclusively of the subset expressing low LFA-3, CD2, LFA-1, CDw29, and UCHL1. Activation of cord peripheral blood mononuclear leukocytes with PHA leads to uniform enhanced expression of each of these molecules on CD3+ cells. Functional analyses of these T cell subsets were performed after sorting of adult T cells based on differential LFA-3 expression. Only the LFA-3+ subset proliferated in response to the Ag tetanus toxoid, even though the LFA-3- subset proliferated more strongly to PHA. Furthermore, the LFA-3+ subset made greater than fivefold more IFN-gamma than the LFA-3- subset in response to PHA, despite the fact that both subsets made equivalent amounts of IL-2. This phenotypic and functional analysis of resting and activated newborn and adult T cells indicates that human memory T cells express enhanced levels of LFA-3, CD2, LFA-1, UCHL1, CDw29, and Pgp-1; we speculate that the increase in expression of T cell adhesion molecules LFA-3, CD2, and LFA-1 on memory cells is functionally important in their enhanced responsiveness.
对参与人类T细胞相互作用的细胞表面分子的研究表明，三种黏附分子（淋巴细胞功能相关抗原3（LFA-3）、CD2和淋巴细胞功能相关抗原1（LFA-1））中每一种的差异表达可将人类外周血T细胞细分为主要亚群。对这些分子与T细胞亚群其他标志物表达之间关系的系统分析表明，人类外周血T淋巴细胞的一个主要亚群的特征是LFA-3、CD2、LFA-1以及其他三种标志物（CDw29 [4B4]、UCHL1和P-糖蛋白1（Pgp-1））的表达增强。UCHL1（29倍）和LFA-3（超过8倍）的相对表达差异很大，而CDw29、CD2、LFA-1和Pgp-1的差异较小（2至4倍）。在CD4⁺细胞、CD8⁺细胞以及B淋巴细胞（CD19⁺）上均发现LFA-3呈双峰分布。新生儿T细胞（CD3⁺）几乎完全由表达低水平LFA-3、CD2、LFA-1、CDw29和UCHL1的亚群组成。用植物血凝素（PHA）激活脐血外周血单个核白细胞会导致这些分子在CD3⁺细胞上均一性表达增强。在根据LFA-3表达差异对成人T细胞进行分选后，对这些T细胞亚群进行了功能分析。只有LFA-3⁺亚群对破伤风类毒素抗原产生增殖反应，尽管LFA-3⁻亚群对PHA的增殖反应更强。此外，尽管两个亚群产生的白细胞介素-2（IL-2）量相当，但LFA-3⁺亚群在对PHA反应时产生的γ干扰素（IFN-γ）比LFA-3⁻亚群多五倍以上。对静息和活化的新生儿及成人T细胞的这种表型和功能分析表明，人类记忆T细胞表达增强水平的LFA-3、CD2、LFA-1、UCHL1、CDw29和Pgp-1；我们推测记忆细胞上T细胞黏附分子LFA-3、CD2和LFA-1表达的增加在其增强的反应性方面具有重要功能。
