Belcher Donald Andrew, Banerjee Uddyalok, Baehr Christopher Michael, Richardson Kristopher Emil, Cabrales Pedro, Berthiaume François, Palmer Andre Francis
William G. Lowrie Department of Chemical and Biomolecular Engineering, College of Engineering, The Ohio State University, Columbus, Ohio, United States of America.
Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, Ohio, United States of America.
PLoS One. 2017 Oct 11;12(10):e0185988. doi: 10.1371/journal.pone.0185988. eCollection 2017.
Pure tense (T) and relaxed (R) quaternary state polymerized human hemoglobins (PolyhHbs) were synthesized and their biophysical properties characterized, along with mixtures of T- and R-state PolyhHbs. It was observed that the oxygen affinity of PolyhHb mixtures varied linearly with T-state mole fraction. Computational analysis of PolyhHb facilitated oxygenation of a single fiber in a hepatic hollow fiber (HF) bioreactor was performed to evaluate the oxygenation potential of T- and R-state PolyhHb mixtures. PolyhHb mixtures with T-state mole fractions greater than 50% resulted in hypoxic and hyperoxic zones occupying less than 5% of the total extra capillary space (ECS). Under these conditions, the ratio of the pericentral volume to the perivenous volume in the ECS doubled as the T-state mole fraction increased from 50 to 100%. These results show the effect of varying the T/R-state PolyhHb mole fraction on oxygenation of tissue-engineered constructs and their potential to oxygenate tissues.
合成了纯紧张态（T）和松弛态（R）的四级结构聚合人血红蛋白（PolyhHb），并对其生物物理性质进行了表征，同时还研究了T态和R态PolyhHb的混合物。观察到PolyhHb混合物的氧亲和力随T态摩尔分数呈线性变化。对肝中空纤维（HF）生物反应器中一根纤维的PolyhHb促进氧合作用进行了计算分析，以评估T态和R态PolyhHb混合物的氧合潜力。T态摩尔分数大于50%的PolyhHb混合物导致缺氧区和高氧区占总毛细血管外空间（ECS）的比例小于5%。在这些条件下，随着T态摩尔分数从50%增加到100%，ECS中中心周围体积与静脉周围体积的比值增加了一倍。这些结果显示了改变T/R态PolyhHb摩尔分数对组织工程构建体氧合作用的影响及其对组织进行氧合的潜力。
