Seki S, Ikeda A, Ishimoto M
Department of Chemical Microbiology, Faculty of Pharmaceutical Sciences, Hokkaido University.
J Biochem. 1988 Apr;103(4):583-4. doi: 10.1093/oxfordjournals.jbchem.a122310.
The NAD(P)H-dependent nitrate reductase system in Clostridium perfringens was reconstituted with rubredoxin (Rd), nitrate reductase (NaR), and an unadsorbed fraction, on a DEAE-cellulose column, of the extract (designated as fraction A), under nitrogen gas. Ferredoxin in place of Rd was not effective as an electron carrier in this reconstituted system. NAD(P)H-dependent nitrate reducing activity was also obtained by replacing fraction A with ferredoxin-NADP+ reductase from spinach. We propose the following scheme for the electron transfer in this NAD(P)H dependent nitrate reduction system. NAD(P)H----NAD(P)H-Rd reductase----Rd----NaR----NO3-.
产气荚膜梭菌中依赖烟酰胺腺嘌呤二核苷酸磷酸（NAD(P)H）的硝酸还原酶系统，在氮气氛围下，于二乙氨基乙基纤维素柱上，用红素氧还蛋白（Rd）、硝酸还原酶（NaR）以及提取物（命名为组分A）的未吸附部分进行了重组。在此重组系统中，用铁氧还蛋白替代Rd作为电子载体无效。通过用菠菜中的铁氧还蛋白 - 烟酰胺腺嘌呤二核苷酸磷酸还原酶替代组分A，也获得了依赖NAD(P)H的硝酸还原活性。我们提出了该依赖NAD(P)H的硝酸还原系统中电子传递的如下方案。NAD(P)H→NAD(P)H - Rd还原酶→Rd→NaR→NO₃⁻ 。
