Liu Zhi-Wei, Li Hui, Wang Wen-Li, Wu Zhi-Jun, Cui Xin, Zhuang Jing
Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University , Nanjing, 210095, People's Republic of China.
J Agric Food Chem. 2017 Nov 8;65(44):9693-9702. doi: 10.1021/acs.jafc.7b04552. Epub 2017 Oct 25.
We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein-protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.
我们采用超高效液相色谱法分析了采后茶叶在高温（38℃）、低温（4℃）和遮荫摊放条件下茶氨酸含量的变化。从相应的蛋白质组数据中鉴定出参与茶氨酸生物合成途径的差异表达蛋白（DEPs），即CsFd - GOGAT和CsNADH - GOGAT。通过酵母双杂交技术验证了CsFd - GOGAT和CsNADH - GOGAT与CsTS1或CsNiR之间的蛋白质 - 蛋白质相互作用。通过定量实时聚合酶链反应分析了包括CsFd - GOGAT和CsNADH - GOGAT在内的17个茶氨酸代谢相关基因的表达谱。分析了茶氨酸含量动态变化与相关基因和DEPs表达谱之间的相关性。本研究初步证明了CsGOGAT在采后茶叶摊放过程中茶氨酸含量动态变化中的重要性。
