J Agric Food Chem. 2019 Jul 3;67(26):7547-7560. doi: 10.1021/acs.jafc.9b01705. Epub 2019 Jun 13.
In this study, isobaric tags for relative and absolute quantitation (iTRAQ) technology were used to investigate three samples from postharvest tea leaves that were treated at room temperature (25 °C, control group), high temperature (38 °C), and low temperature (4 °C) for 4 h. In heat and cold treatments, a total of 635 and 566 differentially expressed proteins (DEPs) were determined, respectively. DEPs were annotated to GO and KEGG databases, which revealed that DEPs involved in various aspects of biological process. Three catechins-related DEPs, CsCHI, CsF3H, and CsANR, were identified. Both catechins contents and the expression profiles of catechins biosynthesis-related genes changed significantly under different temperature treatments. The correlations between catechins contents, gene expression profiles, and DEPs were analyzed. This study provides potential new insights into the molecular basis for tea production of postharvest leaves and catechins content changes at diverse temperature conditions and will guide the improvement of tea-processing technology.
在这项研究中，使用了相对和绝对定量同位素标记（iTRAQ）技术来研究三个样本，这些样本来自于在室温（25°C，对照组）、高温（38°C）和低温（4°C）下处理 4 小时的采后茶叶。在热和冷处理中，分别确定了 635 和 566 个差异表达蛋白（DEPs）。DEPs 被注释到 GO 和 KEGG 数据库中，这表明 DEPs 涉及到生物过程的各个方面。鉴定出了三个儿茶素相关的 DEPs，CsCHI、CsF3H 和 CsANR。在不同温度处理下，儿茶素含量和儿茶素生物合成相关基因的表达谱都发生了显著变化。分析了儿茶素含量、基因表达谱和 DEPs 之间的相关性。这项研究为采后茶叶的分子基础和不同温度条件下儿茶素含量的变化提供了新的见解，并将指导茶叶加工技术的改进。
