Huberman A, Aguilar M B
Department of Biochemistry, Instituto Nacional de la Nutrición Salvador Zubirán, México, D.F.
J Chromatogr. 1988 Jun 29;443:337-42. doi: 10.1016/s0021-9673(00)94805-2.
A crude aqueous extract from 2000 sinus glands of the Mexican crayfish Procambarus bouvieri (Ortmann) was fractionated on a muBondapak-Phenyl column. Two isoforms of the crustacean hyperglycaemic hormone, designated CHH-B and CHH-C in order of elution, were isolated in pure form. Their biochemical characterization showed a remarkable degree of homology. A tryptic digest of each isohormone was fractionated on an Ultrasphere-ODS column. Only one tryptic peptide in CHH-C was eluted later than its homologous peptide in CHH-B. On acid hydrolysis both tryptic peptides had the same composition but, as they contain Asp and Glu, we suspect that the difference residues in a double reciprocal amidation-deamidation of two acidic residues.
从2000个墨西哥螯虾（Procambarus bouvieri，奥尔特曼）的窦腺中提取的粗制水提取物在μBondapak-苯基柱上进行分离。分离出了两种甲壳类高血糖激素同工型，按照洗脱顺序分别命名为CHH-B和CHH-C，均为纯品。它们的生化特性显示出高度的同源性。每种异激素的胰蛋白酶消化产物在Ultrasphere-ODS柱上进行分离。CHH-C中只有一个胰蛋白酶肽段的洗脱时间比CHH-B中其同源肽段晚。酸水解后，两个胰蛋白酶肽段的组成相同，但由于它们含有天冬氨酸和谷氨酸，我们怀疑差异残基存在于两个酸性残基的双倒数酰胺化-脱酰胺化过程中。
