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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Huberman A, Arechiga H, Cimet A, De La Rosa J, Aramburo C

Eur J Biochem. 1979 Aug 15;99(1):203-8. doi: 10.1111/j.1432-1033.1979.tb13246.x.

A neurodepressing hormone has been isolated and purified to homogeneity from aqueous extracts of 2000 eyestalks of the Mexican crayfish Procambarus bouvieri (Ortmann). 2. Purification was achieved by gel filtration on Sephadex G-25 and G-15, and preparative paper electrophoresis at four pH valueimately 1200 molecular weight and composed of neutral amino acids. 5. No N-terminal group could be found. From its electrophoretic behavior it is concluded that the C-terminal group is also blocked.

从墨西哥螯虾（Procambarus bouvieri，奥尔特曼）2000只眼柄的水提取物中分离并纯化出一种神经抑制激素，达到了均一性。2. 通过在Sephadex G - 25和G - 15上进行凝胶过滤，以及在四个pH值下进行制备性纸电泳实现了纯化。5. 未发现N端基团。从其电泳行为推断C端基团也被封闭。

Huberman A, Arechiga H, Cimet A, De La Rosa J, Aramburo C

Eur J Biochem. 1979 Aug 15;99(1):203-8. doi: 10.1111/j.1432-1033.1979.tb13246.x.

A neurodepressing hormone has been isolated and purified to homogeneity from aqueous extracts of 2000 eyestalks of the Mexican crayfish Procambarus bouvieri (Ortmann). 2. Purification was achieved by gel filtration on Sephadex G-25 and G-15, and preparative paper electrophoresis at four pH valueimately 1200 molecular weight and composed of neutral amino acids. 5. No N-terminal group could be found. From its electrophoretic behavior it is concluded that the C-terminal group is also blocked.

从墨西哥螯虾（Procambarus bouvieri，奥尔特曼）2000只眼柄的水提取物中分离并纯化出一种神经抑制激素，达到了均一性。2. 通过在Sephadex G - 25和G - 15上进行凝胶过滤，以及在四个pH值下进行制备性纸电泳实现了纯化。5. 未发现N端基团。从其电泳行为推断C端基团也被封闭。