Shim Ji Seon, Song Min-Young, Yim Sung-Vin, Lee Seung-Eun, Park Kang-Sik
Department of Physiology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Chungcheongbuk-do, Republic of Korea.
J Ginseng Res. 2017 Oct;41(4):566-571. doi: 10.1016/j.jgr.2016.12.003. Epub 2016 Dec 8.
A number of reports have described the protective effects of ginsenoside Rg1 (Rg1) in Alzheimer's disease (AD). However, the protective mechanisms of Rg1 in AD remain elusive.
To investigate the potential mechanisms of Rg1 in β-amyloid peptide-treated SH-SY5Y cells, a comparative proteomic analysis was performed using stable isotope labeling with amino acids in cell culture combined with nano-LC-MS/MS.
We identified a total of 1,149 proteins in three independent experiments. Forty-nine proteins were significantly altered by Rg1 after exposure of the cells to β-amyloid peptides. The protein interaction network analysis showed that these altered proteins were clustered in ribosomal proteins, mitochondria, the actin cytoskeleton, and splicing proteins. Among these proteins, mitochondrial proteins containing HSD17B10, AARS2, TOMM40, VDAC1, COX5A, and NDUFA4 were associated with mitochondrial dysfunction in the pathogenesis of AD.
Our results suggest that mitochondrial proteins may be related to the protective mechanisms of Rg1 in AD.
多项报告描述了人参皂苷Rg1（Rg1）在阿尔茨海默病（AD）中的保护作用。然而，Rg1在AD中的保护机制仍不清楚。
为了研究Rg1在β-淀粉样肽处理的SH-SY5Y细胞中的潜在机制，采用细胞培养中氨基酸稳定同位素标记结合纳升液相色谱-串联质谱法进行了比较蛋白质组学分析。
在三个独立实验中，我们共鉴定出1149种蛋白质。细胞暴露于β-淀粉样肽后，49种蛋白质被Rg1显著改变。蛋白质相互作用网络分析表明，这些改变的蛋白质聚集在核糖体蛋白、线粒体、肌动蛋白细胞骨架和剪接蛋白中。在这些蛋白质中，含有HSD17B10、AARS2、TOMM40、VDAC1、COX5A和NDUFA4的线粒体蛋白与AD发病机制中的线粒体功能障碍有关。
我们的结果表明，线粒体蛋白可能与Rg1在AD中的保护机制有关。
