Pfahler Verena, Tamburini Federica, Bernasconi Stefano M, Frossard Emmanuel
Department of Environmental Systems Science, ETH Zurich, Eschikon 33, 8315 Lindau, Switzerland.
Sustainable Agriculture Sciences, Rothamsted Research, Okehampton, Devon EX20 2SB UK.
Plant Methods. 2017 Sep 25;13:75. doi: 10.1186/s13007-017-0227-x. eCollection 2017.
Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of P and the isotopic composition of oxygen associated to P (δO) in different plant parts of soybean ( cv. Toliman). Two P pools were extracted sequentially with 0.3 M trichloroacetic acid (TCA P) and 10 M nitric acid (HNO; residual P).
The δO of TCA P in the old leaves of the - P plants (23.8‰) significantly decreased compared to the + P plants (27.4‰). The P data point to an enhanced mobilisation of P from residual P in the old leaves of the - P plants compared to the + P plants.
Omitting P for 10 days lead to a translocation of P from source to sink organs in soybeans. This was accompanied by a significant lowering of the δO of TCA P in the source organs due to the enzymatic hydrolysis of organic P. Combining P and δO can provide useful insights in plant responses to P omission at an early stage.
改变磷（P）营养会导致植物代谢发生变化。本研究的目的是探究这些变化如何反映在大豆（品种Toliman）不同植物部位的磷分布以及与磷相关的氧同位素组成（δO）中。用0.3M三氯乙酸（TCA-P）和10M硝酸（HNO；残留磷）依次提取两个磷库。
与施磷（+P）植株相比，缺磷（-P）植株老叶中TCA-P的δO（23.8‰）显著降低。磷数据表明，与施磷植株相比，缺磷植株老叶中残留磷的磷动员增强。
缺磷10天导致大豆中磷从源器官向库器官转移。由于有机磷的酶促水解，源器官中TCA-P的δO显著降低。结合磷和δO可以在早期为植物对缺磷的反应提供有用的见解。
