Rezaei Sajjad, Shamsi Mahdieh Molanouri, Mahdavi Mehdi, Jamali Azadeh, Prestes Jonato, Tibana Ramires Alsamir, Navalta James Wilfred, Voltarelli Fabrício Azevedo
Physical Education and Sport Sciences Department, Faculty of Humanities, Tarbiat Modares University, Jala Ale Ahmad Exp. Tehran, P.O.Box: 14117-13116, Tehran, Iran.
Immunology Department, Pasteur Institute of Iran, Tehran, Iran.
Diabetol Metab Syndr. 2017 Sep 25;9:74. doi: 10.1186/s13098-017-0273-6. eCollection 2017.
Surfactant protein D (SP-D) is a member of the collectin family and is an important component of the pulmonary innate host defense. To find the relationship between exercise training and SP-D in diabetes, we examined the possible effects of a 10-week endurance exercise-training program on serum levels of SP-D, leptin, lipid profile and insulin resistance in obese women with type-2 diabetes (T2DM).
Twenty-two obese women with T2DM were randomly assigned to either exercise training (ET) or control (C) group. A subject dropped from ET group due to personal reasons and 1 subject dropped from C group due to commitment to experiments conditions. A total of twenty obese women with T2DM were randomly assigned into endurance exercise training (ET = 10) and control (C = 10) groups. The training group underwent a progressive endurance-training program for 10 weeks (running on a treadmill for 30-55 min/day at 50-75% heart rate reserve) and the control group did not participate in any exercise program. Venous blood samples were collected from both groups before and 72 h after the last session of exercise training for analysis of serum SP-D, leptin, lipid profile, glucose and insulin. Data were analyzed using 2 (group: control, endurance training) × 2 (time: pre, post) ANOVA with repeated measures on the second factor. Absolute changes from rest (∆ baseline) values were calculated according to the following formula: ((measure-baseline)·baseline-1)·100. Percent change between groups was analyzed using independent t-tests (p < 0.05). All analyses were completed using SPSS 19.
The serum SP-D levels were decreased after exercise training in ET (∆ = - 78.78 ± 17.14%, p = 0.001) when compare to C (∆ = 9.41 ± 4.75%). Obese diabetic women in the ET group showed significantly lower serum leptin levels (8053.27 ± 878.7 pg/ml, ∆ = - 26.97 ± 16.41%) when compared with women in the control group (9885.5 ± 696 pg/ml, ∆ = 7.02 ± 3.46%, p = 0.003). Fasting glucose was favorably and significantly affected by the intervention (ET ∆ = - 17.01 ± 12.74%, Control ∆ = 15.47 ± 7.32%, p = 0.011). VO as an index of aerobic fitness was significantly increased after 10-weeks of endurance exercise training (ET ∆ = 19.29 ± 6.18%).
Endurance exercise training with improvement in aerobic fitness induced a significant reduction of serum SP-D levels in obese women with T2DM.
表面活性蛋白D（SP-D）是凝集素家族的成员，是肺部先天性宿主防御的重要组成部分。为了探究运动训练与糖尿病患者SP-D之间的关系，我们研究了一项为期10周的耐力运动训练计划对2型糖尿病（T2DM）肥胖女性血清中SP-D、瘦素、血脂谱和胰岛素抵抗的可能影响。
22名患有T2DM的肥胖女性被随机分为运动训练（ET）组或对照组（C）。一名受试者因个人原因退出ET组，一名受试者因无法遵守实验条件退出C组。共有20名患有T2DM的肥胖女性被随机分为耐力运动训练组（ET = 10）和对照组（C = 10）。训练组进行了为期10周的渐进性耐力训练计划（在跑步机上以心率储备的50-75%每天跑步30-55分钟），对照组未参与任何运动计划。在运动训练的最后一次训练前和训练后72小时从两组采集静脉血样，用于分析血清SP-D、瘦素、血脂谱、葡萄糖和胰岛素。数据采用2（组：对照组、耐力训练组）×2（时间：训练前、训练后）重复测量方差分析进行分析。根据以下公式计算与静息（∆基线）值的绝对变化：((测量值-基线值)·基线值⁻¹)·100。使用独立t检验分析组间百分比变化（p < 0.05）。所有分析均使用SPSS 19完成。
与C组（∆ = 9.41 ± 4.75%）相比，ET组运动训练后血清SP-D水平降低（∆ = - 78.78 ± 17.14%，p = 0.001）。与对照组女性（9885.5 ± 696 pg/ml，∆ = 7.02 ± 3.46%，p = 0.003）相比，ET组肥胖糖尿病女性的血清瘦素水平显著降低（8053.27 ± 878.7 pg/ml，∆ = - 26.97 ± 16.41%）。干预对空腹血糖有显著的有利影响（ET组∆ = - 17.01 ± 12.74%，对照组∆ = 15.47 ± 7.32%，p = 0.011）。作为有氧适能指标的VO在进行10周耐力运动训练后显著增加（ET组∆ = 19.29 ± 6.18%）。
耐力运动训练可提高有氧适能，使患有T2DM的肥胖女性血清SP-D水平显著降低。
