Ishitani K, Harakawa M, Mikami T, Nagase T, Suzuki S, Suzuki M
Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
J Pharmacobiodyn. 1988 May;11(5):363-8. doi: 10.1248/bpb1978.11.363.
We concluded that a neutral subfraction of mannan from bakers' yeast (Saccharomyces cerevisiae wild type strain), abbreviated as WNM, was able to induce the release of leukotrienes from mouse peritoneal macrophages (M phi). The culture supernatant fluid of M phi from normal mice cultured with WNM caused a marked contraction of guinea pig ileum. This activity was inhibited by pretreatment of M phi with inhibitor of phospholipase A2 or lipoxygenase before M phi were treated with WNM, and by treatment of the incubation bath with a slow reacting substance antagonist, FPL55712. On the other hand, pretreatment of M phi with a cyclooxygenase inhibitor potentiated contractile response of ileum by culture supernatant fluid of M phi. A high performance liquid chromatographic analysis of a culture supernatant fluid of M phi treated with WNM demonstrated the presence of leukotrienes C4 and D4, and their related compounds.
我们得出结论，来自面包酵母（酿酒酵母野生型菌株）的甘露聚糖中性亚组分，简称为WNM，能够诱导小鼠腹腔巨噬细胞（M phi）释放白三烯。用WNM培养的正常小鼠M phi的培养上清液可引起豚鼠回肠明显收缩。在用WNM处理M phi之前，用磷脂酶A2或脂氧合酶抑制剂预处理M phi，以及用慢反应物质拮抗剂FPL55712处理孵育浴，均可抑制这种活性。另一方面，用环氧化酶抑制剂预处理M phi可增强M phi培养上清液对回肠的收缩反应。对用WNM处理的M phi培养上清液进行高效液相色谱分析，结果表明存在白三烯C4和D4及其相关化合物。
