伊拉克木瓜叶乙酸乙酯提取物对乳腺癌和肺癌细胞系的细胞毒性作用。
Cytotoxic Activity of the Ethyl Acetate Extract of Iraqi Carica papaya Leaves in Breast and Lung Cancer Cell Lines.
机构信息
Department of Pharmacognosy, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
Department of Pharmacognosy and Medicinal Plants, Department of Pharmacognosy, College of Pharmacy, University of Baghdad, Iraq.
出版信息
Asian Pac J Cancer Prev. 2023 Feb 1;24(2):581-586. doi: 10.31557/APJCP.2023.24.2.581.
UNLABELLED
The aim of this study was to evaluate the cytotoxic effect of the ethyl acetate fraction of Iraqi Carica papaya (C. papaya) in breast and lung cancer cell lines, MCF-7 and A549, respectively.
METHODS
The ethyl acetate extract of Iraqi C. papaya leaves was prepared and tested for its phytochemical constitution. The 3-(4,5-dimethylthiazoline-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed in breast (MCF-7) and lung (A549) cells lines that were treated with different concentrations of ethyl acetate extract (3.125,6.25,12.5, 25, 50, and 100μg/ml). After 72 hrs of treatment, cell viability was evaluated.
RESULTS
The ethyl acetate extract of C. papaya showed considerable cytotoxic activity in the MCF-7 and A549 cell lines. The activity was dose-dependent; The half-maximum inhibitory concentration (IC50) values were 22.74μg/ml and 8.674 μg/ml in the MCF-7 and A549 cell lines, respectively.
CONCLUSION
The ethyl acetate fraction of Iraqi C. papaya leaves has potential anticancer activity in lung and breast cancer.
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本研究旨在评估伊拉克木瓜（C. papaya）叶的乙酸乙酯提取物在乳腺癌和肺癌细胞系 MCF-7 和 A549 中的细胞毒性作用。
方法
制备伊拉克 C. papaya 叶的乙酸乙酯提取物并测试其植物化学成分。用不同浓度的乙酸乙酯提取物（3.125、6.25、12.5、25、50 和 100μg/ml）处理乳腺癌（MCF-7）和肺癌（A549）细胞系，进行 3-(4,5-二甲基噻唑啉-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定。处理 72 小时后，评估细胞活力。
结果
C. papaya 的乙酸乙酯提取物在 MCF-7 和 A549 细胞系中表现出相当的细胞毒性活性。活性呈剂量依赖性；在 MCF-7 和 A549 细胞系中，半最大抑制浓度（IC50）值分别为 22.74μg/ml 和 8.674μg/ml。
结论
伊拉克木瓜叶的乙酸乙酯部分在肺癌和乳腺癌中具有潜在的抗癌活性。
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