Aljanaby Ahmed Abduljabbar Jaloob, Alhasnawi Haneen Mohammed Reda Jaber
Department of Microbiology, Faculty of Science, University of Kufa, Kufa, Iraq.
Pak J Biol Sci. 2017;20(5):217-232. doi: 10.3923/pjbs.2017.217.232.
Burns infections and urinary tract infections are the most important prevalent diseases in Asian countries, such as Iraq. Klebsiella pneumoniae is one of the most important bacteria cause this type of infections especially in hospitals. Therefore, the aim of this study was to investigate the prevalence of multi-drug resistance K. pneumoniae and extended-spectrum beta-lactamases producing K. pneumoniae isolates from inpatients with urinary tract infection and burns infections in Al-Kufa hospital in Al-Najaf province, Iraq.
A total of 285 clinical samples were collected from in-patients infected with urinary tract infection (141 urine samples) and burns infections (144 burns swabs). Fourteen different antibiotics were used by disc diffusion method and 13 antimicrobials resistance genes were used by PCR technique.
A total of 43 K. pneumoniae strains were isolated. The highest resistance rate was observed for amoxicillin 25 μg and amoxicillin+clavulanic acid 20+10 μg (97.67%) while the lowest resistance rate was observed for imipenem 10 μg (9.30%). The most common resistance associated-genes were blaSHV (86.04%) and at lower prevalence were IMP (9.30%).
Klebsiella pneumoniae strains isolated from burns infections were more virulent than those isolated from urinary tract infections.
烧伤感染和尿路感染是亚洲国家（如伊拉克）最重要的常见疾病。肺炎克雷伯菌是导致这类感染的最重要细菌之一，尤其是在医院环境中。因此，本研究的目的是调查伊拉克纳杰夫省库法医院尿路感染和烧伤感染住院患者中多重耐药肺炎克雷伯菌及产超广谱β-内酰胺酶肺炎克雷伯菌分离株的流行情况。
共收集了285份临床样本，其中包括尿路感染患者的141份尿液样本和烧伤感染患者的144份烧伤拭子样本。采用纸片扩散法检测14种不同抗生素，采用聚合酶链反应技术检测13种抗菌药物耐药基因。
共分离出43株肺炎克雷伯菌菌株。对25μg阿莫西林和20 + 10μg阿莫西林/克拉维酸的耐药率最高（97.67%），而对10μg亚胺培南的耐药率最低（9.30%）。最常见的耐药相关基因是blaSHV（86.04%），IMP的流行率较低（9.30%）。
从烧伤感染中分离出的肺炎克雷伯菌菌株比从尿路感染中分离出的菌株毒性更强。
